Umbelliferone induces changes in the structure and pharmacological activities of Bn IV, a phospholipase A(2) isoform isolated from Bothrops neuwiedi
Autor: | Simone Cristina Buzzo de Oliveira, Benildo Sousa Cavada, Veronica C. G. Soares, Camila Aparecida Cotrim, Sergio Marangoni, Bruno A.M. Rocha, Marcos H. Toyama, Eduardo Britto dos Santos Diz Filho, Daniela de Oliveira Toyama, Plínio Delatorre |
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Rok vydání: | 2010 |
Předmět: |
Male
Platelet Aggregation Myotoxin Molecular Sequence Data Toxicology Umbelliferone chemistry.chemical_compound Phospholipase A2 Animals Edema Bothrops Amino Acid Sequence Umbelliferones Rats Wistar Peptide sequence Phospholipase A biology Base Sequence Circular Dichroism Biological activity Sequence Analysis DNA biology.organism_classification Bothrops neuwiedi Rats Phospholipases A2 Spectrometry Fluorescence Biochemistry chemistry Snake venom biology.protein |
Zdroj: | Toxicon : official journal of the International Society on Toxinology. 57(6) |
ISSN: | 1879-3150 |
Popis: | In this paper was demonstrated that umbelliferone induces changes in structure and pharmacological activities of Bn IV, a lysine 49 secretory phospholipase A(2) (sPLA2) from Bothrops neuwiedi. Incubation of Bn IV with umbelliferone virtually abolished platelet aggregation, edema, and myotoxicity induced by native Bn IV. The amino acid sequence of Bn IV showed high sequence similarities with other Lys49 sPLA2s from B. jararacussu (BthTx-I), B. pirajai (PrTx-I), and B. neuwiedi pauloensis (Bn SP6 and Bn SP7). This sPLA2 also has a highly conserved C-terminal amino acid sequence, which has been shown as important for the pharmacological activities of Lys49 sPLA2. Sequencing of Bn IV previously treated with umbelliferone revealed modification of S(1) and S(20). Fluorescent spectral analysis and circular dichroism (CD) studies showed that umbelliferone modified the secondary structure of this protein. Moreover, the pharmacological activity of Bn IV is driven by synergism of the C-terminal region with the α-helix motifs, which are involved in substrate binding of the Asp49 and Lys49 residues of sPLA2 and have a direct effect on the Ca(2+)-independent membrane damage of some secretory snake venom PLA2. For Bn IV, these interactions are potentially important for triggering the pharmacological activity of this sPLA2. |
Databáze: | OpenAIRE |
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