Dynamic changes of PIRH2 and p27kip1 expression in injured rat sciatic nerve
| Autor: | Tao Chen, Haili Chen, Weipeng Huan, Honghui Li, Guowei Zhang, Biyun Yu, Huibao Hou, Xiang Cao, Jie Deng, Youhua Wang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Nervous system Time Factors Nerve guidance conduit Dermatology Rats Sprague-Dawley Lesion Western blot Neurofilament Proteins Proliferating Cell Nuclear Antigen medicine Animals Immunoprecipitation RNA Messenger Heat-Shock Proteins Cell Proliferation Analysis of Variance biology medicine.diagnostic_test S100 Proteins Nuclear Proteins General Medicine Sciatic nerve injury medicine.disease Sciatic Nerve Nerve Regeneration Rats Cell biology Ubiquitin ligase Disease Models Animal Psychiatry and Mental health Ki-67 Antigen medicine.anatomical_structure Gene Expression Regulation Peripheral nerve injury biology.protein Schwann Cells Neurology (clinical) Sciatic nerve Sciatic Neuropathy medicine.symptom Apoptosis Regulatory Proteins Neuroscience Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Neurological Sciences. 33:749-757 |
| ISSN: | 1590-3478 1590-1874 |
| DOI: | 10.1007/s10072-011-0809-8 |
| Popis: | p53-induced ring-H2 protein (PIRH2), a newly identified E3 ubiquitin ligase, has been reported to be interacted with p27Kip1 and promote ubiquitination of p27Kip1 independently of p53. p27kip1, a member of the Cip/Kip family of cyclin-dependent kinases inhibitors (CKIs), was shown to control cell cycle progression and promote cell proliferation. While the distribution and function of PIRH2 and p27kip1 in nervous system lesion and regeneration remains unclear. Here, we performed a sciatic nerve injury model in adult rats and studied the dynamic changes of PIRH2 and p27kip1 expression by western blot and RT-PCR in injured rat sciatic nerve. Sciatic nerve crush resulted in a significant up-regulation of PIRH2 and a down-regulation of p27kip1. Besides, we observed that they were expressed widely in both Schwann cells and axons in adult rat sciatic nerve by double immunofluorescence staining. Results obtained by coimmunoprecipitation and double labeling further showed their interaction in the regenerating process. Thus, these results indicate that PIRH2 and p27kip1 likely play an important role in peripheral nerve injury and regeneration. |
| Databáze: | OpenAIRE |
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