Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies)
| Autor: | Izabela Dobrowolska, Ilka N. Abreu, Edward Businge, Thomas Moritz, Ulrika Egertsdotter |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Somatic cell Embryo RNA-Seq Germination Plant Science Meristem Biology 01 natural sciences Cell biology Transcriptome 03 medical and health sciences 030104 developmental biology Gene Expression Regulation Plant Botany Shoot Seeds Metabolome Picea 010606 plant biology & botany |
| Zdroj: | Tree physiology. 37(12) |
| ISSN: | 1758-4469 |
| Popis: | Transcriptome, metabolome and histological profiling were performed on normal and aberrant somatic embryo germinants of Norway spruce (Picea abies L. Karst) providing a simplistic systems biology description of conifer germination. Aberrant germinants (AGs) formed periderm-like tissue at the apical pole and lacked shoot growth above the cotyledons. Transcriptome profiling (RNA-Sequencing) revealed a total of 370 differentially expressed genes at ≥1 or ≤-1 log2-fold change, where 92% were down-regulated in AGs compared with normal germinants (NGs). Genes associated with shoot apical meristem formation were down-regulated in AGs, or not differentially expressed between AGs and NGs. Genes involved in hormone signaling and transport were also down-regulated. Metabolite profiling by gas chromatography-mass spectrometry (MS) and liquid chromatography-MS revealed biochemical difference between AGs and NGs, notably increased levels of sugars including glucose in AGs. Genes involved in glucose signaling were down-regulated and genes involved in starch biosynthesis were up-regulated, suggesting involvement of sugar signaling during late embryo development and germination. The overall results provide new data enabling further studies to confirm potential markers for a normal germination process in conifers. |
| Databáze: | OpenAIRE |
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