An IgM specific ELISA for the serodiagnosis of viral bovine respiratory infections
| Autor: | G Florent, A Wiseman |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Paramyxoviridae
medicine.drug_class Immunology Cattle Diseases Enzyme-Linked Immunosorbent Assay Antibodies Viral Monoclonal antibody Immunoglobulin E medicine.disease_cause Respirovirus Infections Microbiology Virus Herpesviridae medicine Animals Immunology and Allergy Infectious Bovine Rhinotracheitis Herpesvirus 1 Bovine General Veterinary biology fungi Antibodies Monoclonal General Medicine biology.organism_classification Virology Respiratory Syncytial Viruses Infectious Diseases Immunoglobulin M Immunoglobulin G Monoclonal biology.protein Cattle Viral disease Antibody |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 13:203-208 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/0147-9571(90)90089-c |
| Popis: | A capture ELISA for the detection of IgM antibodies to Infectious Bovine Rhinotracheitis (IBR) and to Bovine Respiratory Syncytial (BRS) viruses was developed. In these assays, the first monoclonal antibody to bovine IgM is used as the catching antibody while the second monoclonal detects specific antiviral antibodies. The test was evaluated on serum samples originating from both experimentally and naturally infected animals. From these studies, it has been shown that primary IBR and BRS virus infections can be confirmed using serum samples collected 5–10 days after the appearance of the clinical signs of disease. |
| Databáze: | OpenAIRE |
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