Antiproliferative effects of turkish pomegranate (punica granatum l.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS

Autor: Özlem Kurt Şirin, Ahmet C. Gören, Esra Eroglu Ozkan, Oğuz Öztürk, Seda Damla Hatipoglu Cakmar, Tugba Yilmaz Ozden, Ezgi Ersoy, Mehmet Fatih Seyhan, Hülya Yılmaz Aydoğan
Přispěvatelé: Eczacılık Fakültesi, GÖREN, AHMET CEYHAN
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Turkey
Eroglu Ozkan E.
SEYHAN M. F.
Kurt Sirin O.
Yilmaz- Ozden T.
Ersoy E.
Hatipoglu Cakmar S. D.
Goren A. C.
Yilmaz Aydogan H.
Ozturk O.
-Antiproliferative effects of Turkish pomegranate (Punica granatum L.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS-
JOURNAL OF FOOD BIOCHEMISTRY
2021
Cultivated
Cytotoxicity
Cyanidin
Biophysics
Breast Neoplasms
Antioxidants
Pelargonidin
Pomegranate
Anthocyanins
chemistry.chemical_compound
Tandem Mass Spectrometry
Humans
Viability assay
LC-MS/MS
Punicalagin
Pharmacology
Traditional medicine
biology
Plant Extracts
Cell growth
food and beverages
Cell Biology
biology.organism_classification
chemistry
MCF-7
Punica
MCF-7 Cells
Female
Antioxidant
Chromatography
Liquid
Food Science
Popis: In this study, eight different pomegranate (Punica granatum L.) cultivars from Turkey were evaluated for their antioxidant and cytotoxic effects on the MCF-7 breast cancer cell lines and MCF-10A breast fibrocystic epithelial cell lines with a focus on their chemical compositions by LC-MS/MS. Cell lines were treated with pomegranate juice extracts in different doses at selected time intervals (24th, 48th, and 72nd hour). Afterwards, WST-1 cell proliferation assay was performed to investigate the cytotoxicity of the extracts. Accordingly, all extracts decreased the cell viability of MCF-7 breast cancer cell lines and had no cytotoxic effect on the cell viability of MCF-10A cell lines. Among eight extracts, P7 (Izmir 1513), which was rich in anthocyanins such as cyanidin chloride (69.76 +/- 8.02 mu g/g extract), cyanidin-3-O-glucoside (903.66 +/- 101.89 mu g/g extract), and punicalagin (992.09 +/- 174.53 mu g/g extract), was found to demonstrate the strongest cytotoxic activity on MCF-7 breast cancer cell lines by decreasing the cell viability in half at 24th hour with an IC50 value of 49.08 mu g/ml. Practical applications Eight commercially valuable pomegranate (Punica granatum) cultivars from Turkey were examined. Pelargonidin, cyanidin, cyanidin-3-O-gl, callistephin, and delphinidin-3-O-gl were quantified. Two cultivars (P1 and P3) showed comparatively higher antioxidant effects. A cultivar (P7) showed strongest cytotoxic activity against MCF-7 breast cancer cell line. The cultivars have potential to be used as natural antioxidant and anticancer agents.
Databáze: OpenAIRE
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