Covariation of Ergot Severity and Alkaloid Content Measured by HPLC and One ELISA Method in Inoculated Winter Rye across Three Isolates and Three European Countries
| Autor: | Thomas Miedaner, Anna Kodisch, Małgorzata Niewińska, Elisabeth Zechner, Jakob Eifler, Frederik N. Wuppermann, Franz Wieser, Jakub Danielewicz, Brigitta Schmiedchen, Franz Joachim Fromme, Michael Oberforster, Bernd Rodemann, Marek Korbas, Dörthe Siekmann, Andres Gordillo, Anna Tratwal, Armin Raditschnig |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Veterinary medicine Genotype Health Toxicology and Mutagenesis lcsh:Medicine Enzyme-Linked Immunosorbent Assay Food Contamination Biology Toxicology ergot alkaloids 01 natural sciences High-performance liquid chromatography Claviceps Article chemistry.chemical_compound Germany mycotoxins Elisa method Mycotoxin Chromatography High Pressure Liquid Claviceps purpurea Inoculation Alkaloid Secale 010401 analytical chemistry lcsh:R food and beverages biology.organism_classification 0104 chemical sciences rye chemistry Austria ELISA Poland HPLC Edible Grain 010606 plant biology & botany Ergocornine |
| Zdroj: | Toxins Volume 12 Issue 11 Toxins, Vol 12, Iss 676, p 676 (2020) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins12110676 |
| Popis: | Ergot caused by Claviceps purpurea is a problem for food and feed security in rye due to the occurrence of toxic ergot alkaloids (EAs). For grain elevators and breeders, a quick, easy-to-handle, and cheap screening assay would have a high economic impact. The study was performed to reveal (1) the covariation of ergot severity (= percentage of sclerotia in harvested grain) and the content of 12 EAs determined by high performance liquid chromatography (HPLC) and (2) the covariation between these traits and results of one commercial enzyme linked immunosorbent assays (ELISA). In total, 372 winter rye samples consisting of a diverse set of genotypes, locations from Germany, Austria, and Poland over two years, and three isolates were analyzed. Ergocornine and &alpha ergocryptine were detected as major EAs. Ergocristinine occurred as a minor component. Claviceps isolates from different countries showed a similar EA spectrum, but different quantities of individual EAs. A moderate, positive covariation between ergot severity and EA content determined by HPLC was observed across two years (r = 0.53, p < 0.01), but large deviation from the regression was detected. ELISA values did neither correlate with the HPLC results nor with ergot severity. In conclusion, a reliable prediction of the EA content based on ergot severity is, at present, not possible. |
| Databáze: | OpenAIRE |
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