GATA factor-dependent regulation of cardiac m2 muscarinic acetylcholine gene transcription
| Autor: | Neil M. Nathanson, Marc L. Rosoff |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Transcriptional Activation
animal structures Transcription Genetic Recombinant Fusion Proteins Response element Chick Embryo Biology Transfection Biochemistry Cell Line Transactivation Muscarinic acetylcholine receptor M5 Muscarinic acetylcholine receptor Animals Amino Acid Sequence Promoter Regions Genetic Molecular Biology Transcription factor Cells Cultured Conserved Sequence Receptor Muscarinic M2 Myocardium Muscarinic acetylcholine receptor M2 Promoter Zinc Fingers Cell Biology Molecular biology Receptors Muscarinic DNA-Binding Proteins Gene Expression Regulation embryonic structures COS Cells GATA transcription factor Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 273(15) |
| ISSN: | 0021-9258 |
| Popis: | The m2 subtype is the predominant muscarinic acetylcholine receptor subtype expressed in heart and regulates the rate and force of cardiac contraction. We have previously reported the isolation of the promoter region for the chick m2 receptor gene and defined a region of the chick m2 promoter sufficient for high level expression in cardiac primary cultures. In this manuscript we demonstrate transactivation of cm2 promoter by the GATA family of transcription factors. In addition, we define the GATA-responsive element in the chick m2 promoter and demonstrate that this element is required for expression in cardiac primary cultures. Finally, we demonstrate specific binding of both a chick heart nuclear protein and of cloned chick GATA-4, -5, and -6 to the GATA-responsive element. |
| Databáze: | OpenAIRE |
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