Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminant from a known renal carcinoma cell line
| Autor: | Michael Stürzl, Don Ganem, Ramona Jochmann, Wilhelm G. Dirks, Dominika Gaus |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Biology medicine.disease Immunohistochemistry Kidney Neoplasms Pathogenesis Cytokeratin Oncology Cell culture Cell Line Tumor Cancer research medicine Carcinoma Humans Sarcoma Carcinoma Renal Cell Sarcoma Kaposi Kaposi's sarcoma Immortalised cell line Microsatellite Repeats |
| Zdroj: | International Journal of Cancer. 132:1954-1958 |
| ISSN: | 0020-7136 |
| DOI: | 10.1002/ijc.27849 |
| Popis: | Kaposi's sarcoma (KS) is an endothelial cell-derived tumor. Investigations of the molecular mechanisms of KS pathogenesis and the identification of drugs for treatment of KS depend critically on valid cell-culture models. Two major immortalized cell lines are available for KS research. Recently, the KS cell line KS Y-1 has been shown to be cross-contaminated with the T24 urinary bladder cancer cell line (ATCC HTB-4). Here, we show by short tandem repeat profiling that the second KS cell line, SLK, is indistinguishable from the clear-cell renal-cell carcinoma cell line Caki-1. Immunocytochemical detection of cytokeratin expression confirmed the epithelial-cell origin of SLK cells. Our findings indicate that SLK cells are not of endothelial origin and should not be used in future studies as a model for KS-derived endothelial tumor cells. We suggest that in the future, more attention needs to be paid to the authenticity of cells in lines derived from human tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text