A vaccine based on a mutant transferrin binding protein B of Haemophilus parasuis induces a strong T-helper 2 response and bacterial clearance after experimental infection
| Autor: | M. J. García-Iglesias, Álvaro Álvarez-Estrada, Rafael Frandoloso, E. F. Rodríguez-Ferri, Claudia Pérez-Martínez, Sonia Martínez-Martínez, C.B. Gutiérrez-Martín |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Haemophilus Infections Swine Immunology Spleen Microbiology Transferrin-Binding Protein B 03 medical and health sciences Haemophilus parasuis 0302 clinical medicine Immune system Th2 Cells Haemophilus medicine Animals Haemophilus Vaccines chemistry.chemical_classification Swine Diseases General Veterinary biology Binding protein Vaccination biology.organism_classification Virology Antibodies Bacterial 030104 developmental biology medicine.anatomical_structure chemistry Transferrin Mutation biology.protein Cytokines Antibody 030215 immunology |
| Zdroj: | Veterinary immunology and immunopathology. 179 |
| ISSN: | 1873-2534 |
| Popis: | This study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutant Haemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin. Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophages and spleen after the vaccination and challenge of twenty-two colostrum-deprived pigs with 10(8) CFU of H. parasuis were analysed. Pigs vaccinated with Y167A were compared to those vaccinated with native TbpB (nTbpB), those treated with a commercial bacterin (CB) against Glasser's disease, those unvaccinated challenged (CH) and those unvaccinated unchallenged (UNCH) pigs. The rectal temperatures of Y167A pigs resembled those of UNCH pigs and were significantly lower than those of the nTbpB, CB and CH animals. A major reduction in pathological changes of the challenged pigs was observed in the Y167A group. H. parasuis was cleared from 88.9% of the samples from Y167A pigs versus 60.0% and 55.6% from those of the CB and nTbpB groups, respectively. The antibody response elicited by Y167A by ELISA was notably higher than that observed for nTbpB and CB pigs and was capable of preventing the expression and secretion of IL-8. The expression of IL-4 and IL-5, which were associated with the specific antibody levels, suggests that the main mechanism of protection conferred by Y167A vaccine is based on a strong T-helper 2 response. |
| Databáze: | OpenAIRE |
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