| Popis: |
PDF file - 1MB, Depletion of GSH and production of ROS may contribute to STC-induced apoptosis in human leukemic and colorectal cancer cells. (A) HL-60, K562, HT-29, and SNU-C4 cells were treated with STC (0.3, 0.5 muM) or STC (1.0, 1.5 muM) for 2 h, 2h, 24 h, and 24 h, respectively, in the presence or absence of GSH, NAC, catalase, or BSO and measured intracellular GSH content using glutathione assay kit. **, P < 0.01; ***, P < 0.001. (B) HL-60, K562, HT-29, and SNU-C4 cells (1 �~ 105 cells/ well) were incubated with STC in the presence or absence of GSH, NAC, catalase, or BSO for 6 h, 6 h, 48 h, and 48 h, respectively. After treatment for the indicated times, the percentage of apoptotic cells was determined by annexin V-FITC/PI staining as described in the Materials and Methods section. Data show the mean �} SD of 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) HL-60, K562, HT-29, and SNU-C4 cells (1 �~ 105 cells/ well) were incubated with STC in the presence or absence of GSH, NAC, catalase, or BSO for 2 h, 2 h, 24 h, and 24 h, respectively, after which they were labeled with an oxidant-sensitive dye (DCF-DA) and analyzed by flow cytometry. **, P < 0.01; ***, P < 0.001, yielding significantly lower values than those obtained for cells treated with STC in the absence of GSH, NAC, or catalase. |
