Comparison of Neurological Function in Males and Females from Two Substrains of C57BL/6 Mice
| Autor: | Helen Garber, Amy Ashworth, Molly S. Griffith, Christine Perdan Curran, Jocelyn Phillips Fowler, Mark E. Bardgett |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Gerontology
C57BL/6 sex differences Health Toxicology and Mutagenesis Neurological function Physiology Male mice Morris water navigation task Context (language use) Toxicology lcsh:Chemical technology Locomotor activity Open field Article Medicine lcsh:TP1-1185 genetics Fear conditioning substrain Chemical Health and Safety biology business.industry behavior neurobiology biology.organism_classification 3. Good health business |
| Zdroj: | Toxics Volume 3 Issue 1 Pages 1-17 Toxics, Vol 3, Iss 1, Pp 1-17 (2014) |
| ISSN: | 2305-6304 |
| DOI: | 10.3390/toxics3010001 |
| Popis: | The C57BL/6 (B6) mouse is the background strain most frequently used for genetically-modified mice. Previous studies have found significant behavioral and genetic differences between the B6J (The Jackson Laboratory) and B6N substrains (National Institutes of Health) however, most studies employed only male mice. We performed a comprehensive battery of motor function and learning and memory tests on male and female mice from both substrains. The B6N male mice had greater improvement in the rotarod test. In contrast, B6J female mice had longer latencies to falling from the rotarod. In the Morris water maze (MWM), B6J males had significantly shorter latencies to finding the hidden platform. However, B6N females had significantly shorter path lengths in the reversal and shifted-reduced phases. In open field locomotor activity, B6J males had higher activity levels, whereas B6N females took longer to habituate. In the fear conditioning test, B6N males had a significantly longer time freezing in the new context compared with B6J males, but no significant differences were found in contextual or cued tests. In summary, our findings demonstrate the importance of testing both males and females in neurobehavioral studies. Both factors (sex and substrain) must be taken into account when designing developmental neurotoxicology studies. |
| Databáze: | OpenAIRE |
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