Injectable biomimetic liquid crystalline scaffolds enhance muscle stem cell transplantation
| Autor: | Charles M. Rubert Pérez, Benjamin D. Cosgrove, Samuel I. Stupp, Adam T. Preslar, Russell D. Haynes, Thomas J. Meade, Charlotte H. Chen, M. Hussain Sangji, Helen M. Blau, Mark T. McClendon, Eduard Sleep |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Cellular differentiation Population Nanofibers 02 engineering and technology Myoblasts Mice 03 medical and health sciences Biomimetic Materials medicine Animals Myocyte Progenitor cell Muscle Skeletal education education.field_of_study Multidisciplinary Tissue Scaffolds Regeneration (biology) Graft Survival Skeletal muscle 021001 nanoscience & nanotechnology Liquid Crystals Cell biology Transplantation 030104 developmental biology medicine.anatomical_structure PNAS Plus Stem cell 0210 nano-technology Stem Cell Transplantation Biomedical engineering |
| Zdroj: | Proceedings of the National Academy of Sciences. 114 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1708142114 |
| Popis: | Muscle stem cells are a potent cell population dedicated to efficacious skeletal muscle regeneration, but their therapeutic utility is currently limited by mode of delivery. We developed a cell delivery strategy based on a supramolecular liquid crystal formed by peptide amphiphiles (PAs) that encapsulates cells and growth factors within a muscle-like unidirectionally ordered environment of nanofibers. The stiffness of the PA scaffolds, dependent on amino acid sequence, was found to determine the macroscopic degree of cell alignment templated by the nanofibers in vitro. Furthermore, these PA scaffolds support myogenic progenitor cell survival and proliferation and they can be optimized to induce cell differentiation and maturation. We engineered an in vivo delivery system to assemble scaffolds by injection of a PA solution that enabled coalignment of scaffold nanofibers with endogenous myofibers. These scaffolds locally retained growth factors, displayed degradation rates matching the time course of muscle tissue regeneration, and markedly enhanced the engraftment of muscle stem cells in injured and noninjured muscles in mice. |
| Databáze: | OpenAIRE |
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