Maturation of human dendritic cells by monocyte-conditioned medium is dependent upon trace amounts of lipopolysaccharide inducing tumour necrosis factor α
| Autor: | Vagn Andersen, Morten Svenson, Jacob Nersting, Klaus Bendtzen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipopolysaccharides
Necrosis Lipopolysaccharide Cell Survival medicine.medical_treatment Immunology Immunoglobulins Stimulation Inflammation Biology Monocytes chemistry.chemical_compound Antigens CD medicine Humans Immunology and Allergy Inducer Cells Cultured Membrane Glycoproteins Tumor Necrosis Factor-alpha Monocyte Antibodies Monoclonal Cell Differentiation Dendritic Cells HLA-DR Antigens Dendritic cell Flow Cytometry Cell biology medicine.anatomical_structure chemistry Biochemistry Culture Media Conditioned B7-2 Antigen medicine.symptom Prostaglandin E |
| Zdroj: | Immunology Letters. 89:59-65 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/s0165-2478(03)00110-x |
| Popis: | We investigated the ability of monocyte-conditioned medium (MCM), generated by monocytes cultured on plastic-immobilised immunoglobulin, to stimulate maturation of human monocyte-derived dendritic cells (DC). Earlier reports suggest that MCM is a strong inducer of irreversible DC maturation, whereas we find, that adding a small amount of lipopolysaccharide (LPS) to the MCM-generating cultures is required for the production of a DC-stimulatory MCM. Moreover, compared with addition of LPS directly to the DC cultures, stimulation via MCM cultures increases by several fold the DC-stimulatory potency of LPS. Maturation by this procedure is mediated mainly by tumour necrosis factor alpha secreted from monocytes during the medium-conditioning period. |
| Databáze: | OpenAIRE |
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