Targeted disruption of the Mn1 oncogene results in severe defects in development of membranous bones of the cranial skeleton
| Autor: | Ellen C. Zwarthoff, Magda A. Meester-Smoor, Karel H. M. van Wely, A. C. P. Hekman, Marcel Vermeij, Christl Vermey-Keers, Marjolein J. L. van Helmond, Anco C. Molijn, Peter Riegman |
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| Přispěvatelé: | Pathology |
| Rok vydání: | 2005 |
| Předmět: |
Aging
Heterozygote Longevity Vomer Biology Meningioma Exon Mice medicine Mammalian Genetic Models with Minimal or Complex Phenotypes Animals Molecular Biology Mice Knockout Oncogene Proteins Tumor Suppressor Proteins Homozygote Skull Myeloid leukemia Cell Biology Anatomy medicine.disease Survival Analysis Cleft Palate ETV6 medicine.anatomical_structure Animals Newborn Organ Specificity Intramembranous ossification Knockout mouse Trans-Activators Head Gene Deletion |
| Zdroj: | Molecular and Cellular Biology, 25(10), 4229-4236. American Society for Microbiology |
| ISSN: | 0270-7306 |
| Popis: | Fusion of the MN1 gene to TEL (ETV6) results in myeloid leukemia. The fusion protein combines the transcription activating domain of MN1 and the DNA binding domain of TEL and is thought to act as a deranged transcription factor. In addition, disruption of the large first exon of the MN1 gene is thought to inactivate MN1 function in a meningioma. To further investigate the role of MN1 in cancer, we generated Mn1 knockout mice. Mn1(+/-) animals were followed for 30 months, but they had no higher incidence of tumor formation than wild-type littermates. Mn1 null mice, however, were found to die at birth or shortly thereafter as the result of a cleft palate. Investigation of newborn or embryonic day 15.5 (E15.5) to E17.5 null mice revealed that the development of several bones in the skull was abnormal. The affected bones are almost exclusively formed by intramembranous ossification. They are either completely agenic at birth (alisphenoid and squamosal bones and vomer), hypoplastic, deformed (basisphenoid, pterygoid, and presphenoid), or substantially thinner (frontal, parietal, and interparietal bones). In heterozygous mice hypoplastic membranous bones and incomplete penetrance of the cleft palate were observed. We conclude that Mn1 is an important factor in development of membranous bones. |
| Databáze: | OpenAIRE |
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