Comparison of stem cell viability of bone marrow cryopreserved by two different methods
| Autor: | Ling-Yang Chen, Lai-Lu Chong, Chi-Kuan Ho, Sheng-Yuan Wang, Po-Min Chen, Chien-Hui Yung |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Cell Survival
Bone Marrow Cells Biology Hydroxyethyl starch General Biochemistry Genetics and Molecular Biology Cryopreservation Colony-Forming Units Assay Andrology Mice chemistry.chemical_compound Cryoprotective Agents Freezing medicine Animals Viability assay Dimethyl sulfoxide Organ Preservation General Medicine Hematopoietic Stem Cells medicine.anatomical_structure chemistry Immunology Trypan blue Bone marrow Stem cell General Agricultural and Biological Sciences Stem Cell Recovery medicine.drug |
| Zdroj: | Cryobiology. 24:229-237 |
| ISSN: | 0011-2240 |
| Popis: | Two different cryogenic methods were used to study the preservation of murine bone marrow cells. Compared to the classical methods, in which separated mononuclear marrow cells in 10% dimethyl sulfoxide (DMSO) were cryopreserved in liquid nitrogen (-196 degrees C), a modified technique was carried out by cryopreservation of unfractionated marrow cells in a mixed protectant of 5% DMSO and 6% hydroxyethyl starch (HES) at -80 degrees C. Samples that were separately thawed after storage for 1, 4, 8, and 12 weeks were assayed for cell viability and recovery of CFU-GM and CFU-S. No macroscopic clumping of cells was noted either in fractionated or in unfractionated marrow cell cryopreservations. A mild damage, about 25% reduction of stem cells, was found at 1 week and did not deepen further. It seems that the greatest loss of stem cells occurred in the process of cryopreservation itself. Compared to prefreeze values, both a high number of cells that excluded trypan blue (87 +/- 3.4%) and a high recovery of CFU-GM (75 +/- 9.8%) and CFU-S (74 +/- 11.2) were observed in unfractionated marrow samples cryopreserved with the DMSO/HES mixture at -80 degrees C for 3 months and these results were very similar to those obtained from fractionated mononuclear marrow cells cryopreserved at -196 degrees C. The DMSO/HES protectant provides a simplified bone marrow cryopreservation technique that should be favorable to clinical application because of its high stem cell recovery and avoidance of cell-separation manipulation. |
| Databáze: | OpenAIRE |
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