Early exposure to caffeine affects gene expression of adenosine receptors, DARPP-32 and BDNF without affecting sensibility and morphology of developing zebrafish (Danio rerio)
| Autor: | Julhana Bianchini Pohlmann, Giovanna Medeiros Tavares de Oliveira, Rosane Souza da Silva, Lidiane Fazenda, Maurício Reis Bogo, Katiucia Marques Capiotti, Luiza Reali Nazario, Fabiano Peres Menezes, Carla Denise Bonan |
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| Rok vydání: | 2011 |
| Předmět: |
Embryo
Nonmammalian Gene Expression Developmental/drug effects Toxicology Caffeine/pharmacology chemistry.chemical_compound Neurotrophic factors Receptors Behavior Animal/drug effects Receptor Zebrafish Embryonic Development/drug effects Touch/drug effects biology Behavior Animal Brain-Derived Neurotrophic Factor/genetics Gene Expression Regulation Developmental Animal/drug effects Purinergic signalling Cell biology Embryo adenosine Purinergic P1/genetics Drug Caffeine medicine.drug medicine.medical_specialty Dopamine and cAMP-Regulated Phosphoprotein 32 animal structures Nonmammalian/drug effects Embryo Nonmammalian/drug effects Embryonic Development Gene Expression/drug effects Adenosinergic Dose-Response Relationship Cellular and Molecular Neuroscience Developmental Neuroscience Internal medicine medicine Animals adenosine A1 receptors Behavior Gene Expression Regulation Developmental/drug effects Dose-Response Relationship Drug Brain-Derived Neurotrophic Factor Receptors Purinergic P1 adenosine A2A receptors Receptors Purinergic P1/genetics zebrafish biology.organism_classification Adenosine Adenosine receptor Endocrinology Gene Expression Regulation chemistry Touch Zebrafish/embryology Dopamine and cAMP-Regulated Phosphoprotein 32/genetics |
| Zdroj: | Repositório Institucional PUCRS Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS Neurotoxicology and Teratology, 33(6), 680-5 |
| ISSN: | 1872-9738 0892-0362 |
| Popis: | Adenosine receptors are the most important biochemical targets of caffeine, a common trimethylxanthine found in food and beverages. Adenosine plays modulatory action during the development through adenosine receptors and their intracellular pathways activation. In this study, we aimed to evaluate if caffeine gave to zebrafish in the very first steps of development is able to affect its direct targets, through the adenosine receptors mRNA expression evaluation, and latter indirect targets, through evaluation of the pattern of dopamine and cAMP-regulated phosphoprotein and brain-derived neurotrophic factor (BDNF) mRNA expression. Here, we demonstrate that zebrafish express adenosine receptor subtypes (A1, A2A1, A2A2 and A2B) since 24 h post-fertilization (hpf) and that caffeine exposure is able to affect the expression of these receptors. Caffeine exposure from 1 hpf is able to increase A1 expression at 72–96 hpf and A2A1 expression at 72 hpf. No alterations occurred in A2A2 and A2B expression after caffeine treatment. DARPP-32, a phosphoprotein involved in adenosine intracellular pathway is also expressed since 24 hpf and early exposure to caffeine increased DARPP-32 expression at 168 hpf. We also evaluate the expression of BDNF as one of the targets of adenosine intracellular pathway activation. BDNF was also expressed since 24 hpf and caffeine treatment increased its expression at 48 and 72 hpf. No morphological alterations induced by caffeine treatment were registered by the check of general body features and total body length. Assessment of tactile sensibility also demonstrated no alterations by caffeine treatment. Altogether, these results suggest that caffeine is able to affect expression of its cellular targets since early phases of development in zebrafish without affect visible features. The up-regulation of direct and indirect targets of caffeine presents as a compensatory mechanism of maintenance of adenosinergic modulation during the developmental phase. |
| Databáze: | OpenAIRE |
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