Identification of calcium stress induced genes in amaranth leaves through suppression subtractive hybridization
| Autor: | Hugo S. Aguilar-Hernández, Ana P. Barba de la Rosa, Antonio De León-Rodríguez, Alberto Barrera-Pacheco, Leticia Santos, Ramón G. Guevara-González, Eduardo Espitia-Rangel, Fabiola León-Galván |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Transcriptional Activation
Physiology Molecular Sequence Data Down-Regulation Plant Science Genes Plant Gene Expression Regulation Plant Stress Physiological Complementary DNA Amino Acid Sequence RNA Messenger Gene Transcription factor Phylogeny Gene Library Plant Proteins Zinc finger Regulation of gene expression Amaranthus Wall-Associated Kinase Base Sequence biology Nucleic Acid Hybridization Zinc Fingers Sequence Analysis DNA Molecular biology Up-Regulation Cell biology Plant Leaves RNA Plant Seedlings Suppression subtractive hybridization biology.protein Calcium Signal transduction Sequence Alignment Agronomy and Crop Science Signal Transduction Transcription Factors |
| Zdroj: | Journal of Plant Physiology. 168:2102-2109 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2011.06.006 |
| Popis: | Calcium (Ca 2+ ) is a critical ion for the growth and development of plants and plays an important role in signal transduction pathways in response to biotic and abiotic stresses. We investigated the Ca 2+ stress responsive-genes in amaranth leaves by using the suppression subtractive hybridization technique. Screening of the libraries generated 420 up-regulated transcripts and 199 down-regulated transcripts. The differentially expressed transcripts were associated with general stress response, transcription factors, gene regulation, signal transduction, and some other with unknown function. Selected genes were used to study their differential regulation by sqRT-PCR. Among the up-regulated transcripts, a fragment containing the motif of C3HC4-type RING-Zinc family was further characterized. The ORF of amaranth zinc finger protein ( Ah Znf) has a closer relationship with its ortholog from Ricinus communis while is distantly related to the Arabidopsis thaliana C3HC4-type ortholog. We have identified a novel putative zinc finger protein along with other novel proteins such as the wall associated kinase, phosphoinositide binding protein, and rhomboid protease involved in response to Ca 2+ stress in amaranth leaves. |
| Databáze: | OpenAIRE |
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