Value of Urease Test in the detection of Helicobacter pylori infections
| Autor: | Mohammad Rakhshan, Hossein Eliasi, Faransis Megraud, Ahad Zargarizadeh, Hossein Goodarzi, Reza Gottaslou, Bahram Kazemi |
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| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | فصلنامه دانشگاه علوم پزشکی جهرم, Vol 2, Iss 2, Pp 6-11 (2005) |
| ISSN: | 2008-8442 2008-7993 |
| DOI: | 10.29252/jmj.2.2.3 |
| Popis: | Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus diagnosis and treatment of H.pylori infection is very important. Material and Methods : The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pyolri was detcted in gastric mucosal biopsy specimens by Biopsy Urease Test, PCR and histopathology. A media was used for urease test, Normal Broth Urea. The biopsy is placed in a broth containing urea and a PH indicator. The goldstandard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C (294 bp). Results : The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR. Conclusion : The result of This study showed that urease test was not a sensitive and specific method for accurate detection of the infection. It a level of the sensitivity is too low to be used as the sole method of detection in ordinary practic. In this situation other/ tests should be used. |
| Databáze: | OpenAIRE |
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