One-step immobilization of antibodies and DNA on gold sensor surfaces via a poly-adenine oligonucleotide approach
| Autor: | César S. Huertas, Laura M. Lechuga, Maria Soler, M.-Carmen Estevez |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission |
| Rok vydání: | 2020 |
| Předmět: |
Point of care diagnostic
Surface Properties Oligonucleotides One-Step Biosensing Techniques 010402 general chemistry 01 natural sciences Antibodies Analytical Chemistry Surface Functionalization chemistry.chemical_compound Plasmonic biosensors Biorecognition elements Background signals Particle Size biology Oligonucleotide 010401 analytical chemistry Limits of detection Conventional methods 0104 chemical sciences Biomarker chemistry Biochemistry biology.protein Gold Antibody DNA Probes Poly A Biosensor Biomarkers DNA Cost-effective solutions |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Label-free plasmonic biosensors have demonstrated promising capabilities as analytical tools for the detection of virtually any type of biomarker. They are presented as good candidates for precision diagnostics since they offer highly sensitive, cost-effective solutions that can be used in any clinical or laboratory setting without the need for specialized trainees. However, different surface functionalization protocols are required, depending on the nature of the biorecognition element, limiting their capabilities for integrated multi-biomarker detection. Here, we present a simple, yet efficient, one-step immobilization approach that is common for both DNA probes and antibodies. Our immobilization approach relies on the incorporation of poly-adenine (polyA) blocks in both nucleic acid probes and antibodies. PolyA sequences have a remarkable affinity for gold surfaces and can specifically interact with sufficient strength to generate stable, dense, and highly ordered monolayers. We have demonstrated excellent performance of our universal functionalization method, showing limits of detection and quantification in the pM–nM range. Moreover, it was able to reduce up to 50% of the background signal from undiluted serum samples compared to conventional methods, demonstrating the immense potential of this strategy for the direct analysis of human biofluids, essential for rapid point-of-care diagnostics. The polyA-based immobilization approach is a promising alternative for the generation of multiplexed biosensors that can detect both protein and nucleic acid biomarkers for multiparametric diagnostic assays. This work has made use of the Biodeposition and Biodetection Unit (U4) from ICTS NANBIOSIS partially supported by MICINN/FEDER (FICTS-1420-27 selected by MICINN). ICN2 is the recipient of Grant SEV-2017-0706 from the “Severo Ochoa Centers of Excellence” Program of Spanish Ministry of Science. C.S.H. is a recipient of an RMIT Vice Chancellor’s Postdoctoral Fellowship. |
| Databáze: | OpenAIRE |
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