An Abundant Periplasmic Protein of the Denitrifying Phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a Component of an ABC Phosphate Transport System
| Autor: | Toshio Satoh, Satomi Hara, Yoshiko Iwasaki, Masahiro Matsuzaki, Isamu Yamamoto, Miho Abe |
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| Rok vydání: | 2003 |
| Předmět: |
Physiology
Molecular Sequence Data Malates lac operon Rhodobacter sphaeroides Plant Science medicine.disease_cause Phosphates Bacterial Proteins medicine Phosphate Transport Proteins Amino Acid Sequence Escherichia coli Peptide sequence Sinorhizobium meliloti Rhodobacter Sequence Homology Amino Acid biology Cell Biology General Medicine Periplasmic space biology.organism_classification Biochemistry Regulatory sequence ATP-Binding Cassette Transporters Periplasmic Proteins |
| Zdroj: | Plant and Cell Physiology. 44:212-216 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcg021 |
| Popis: | To understand a physiological role of an abundant 34-kDa periplasmic protein in the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans grown in a medium containing malate as the carbon source, the gene for the protein was isolated. The deduced amino acid sequence of the protein had a sequence similarity of 66.2% to that of PstS from Sinorhizobium meliloti. The downstream sequence of the Rhodobacter pstS contained five genes similar to pstCAB and phoUB, and its upstream sequence contained a putative regulatory sequence that is analogous to the Pho box involved in phosphate-limitation-induced gene expression in Escherichia coli. Both the amount of the PstS and the pstS promoter-driven expression of lacZ activity increased about two-fold in response to phosphate limitation. This is the first isolation of pst genes encoding proteins of an ABC phosphate transporter system from phototrophic bacteria. |
| Databáze: | OpenAIRE |
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