Thymosin beta-4 upregulates anti-oxidative enzymes and protects human cornea epithelial cells against oxidative damage
| Autor: | K-C Tseng, Y Su, J H-C Ho, W-H Ma, K-H Chen, O K-S Lee |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Apoptosis
Antioxidants Cellular and Molecular Neuroscience Downregulation and upregulation medicine Humans Viability assay Enzyme Inhibitors Cells Cultured chemistry.chemical_classification Reactive oxygen species biology Reverse Transcriptase Polymerase Chain Reaction Epithelium Corneal Thymosin Hydrogen Peroxide Catalase Recombinant Proteins Sensory Systems Epithelium Up-Regulation Cell biology Enzyme Activation Thymosin beta-4 Oxidative Stress Ophthalmology medicine.anatomical_structure Biochemistry chemistry biology.protein Intracellular |
| Zdroj: | British Journal of Ophthalmology. 92:992-997 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.2007.136747 |
| Popis: | Background: The ability to scavenge reactive oxygen species (ROS) is crucial for cornea epithelial cells to resist oxidative damage. The authors previously demonstrated that exogenous thymosin beta-4 (Tβ 4 ) was able to protect human cornea epithelial (HCE-T) cells against H 2 O 2 -induced oxidative damage, and its cellular internalisation was essential. The aim of this study is to further elucidate its protective mechanism. Methods: HCE-T cells with or without Tβ 4 pretreatment were exposed to H 2 O 2 , and the differences in caspase activity, intracellular ROS levels, cell viability, and the expression of anti-oxidative enzymes, were measured and compared. Results: Besides reducing caspase-9 activation and intracellular ROS levels induced by H 2 O 2 , treatment of Tβ 4 could also increase cell viability and stimulate the expression of manganese superoxide dismutase (SOD) and copper/zinc SOD. Moreover, both transcription and translation levels of catalase were also upregulated by Tβ 4 in the presence of exogenous H 2 O 2 . Furthermore, it was demonstrated that the addition of catalase inhibitor abrogated the protective effect of Tβ 4 against H 2 O 2 -induced oxidative damage. Conclusion: To the best of the authors9 knowledge, this is the first report to show that Tβ 4 was capable of upregulating anti-oxidative enzymes in human corneal epithelial cells, and these findings further support its role in cornea protection. |
| Databáze: | OpenAIRE |
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