Mycobacterium tuberculosis phoP mutant: lipoarabinomannan molecular structure
| Jazyk: | English |
|---|---|
| ISSN: | 1350-0872 1465-2080 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3f65160e6481c80b5d7675409baf8e6 https://hal.science/hal-00177982 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....c3f65160e6481c80b5d7675409baf8e6 |
| Autor: | Carlos Martin, Pascal Ludwiczak, Martine Gilleron, Germain Puzo, Brigitte Gicquel, Yann Bordat |
| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Génétique mycobactérienne - Mycobacterial genetics, Institut Pasteur [Paris] (IP), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut Pasteur [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Lipopolysaccharides
MESH: Signal Transduction MESH: Electrophoresis Capillary Magnetic Resonance Spectroscopy MESH: Mycobacterium tuberculosis Acylation Mutant medicine.disease_cause Phosphatidylinositols chemistry.chemical_compound MESH: Bacterial Proteins 0303 health sciences Mutation MESH: Gene Expression Regulation Bacterial biology [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Adaptation Physiological 3. Good health Biochemistry Signal Transduction Tuberculosis MESH: Mutation Virulence Microbiology Gas Chromatography-Mass Spectrometry Mycobacterium tuberculosis 03 medical and health sciences Bacterial Proteins medicine MESH: Phosphatidylinositols Humans Phosphatidylinositol 030304 developmental biology MESH: Acylation Lipoarabinomannan MESH: Humans 030306 microbiology MESH: Magnetic Resonance Spectroscopy Electrophoresis Capillary Gene Expression Regulation Bacterial biology.organism_classification medicine.disease MESH: Adaptation Physiological MESH: Gas Chromatography-Mass Spectrometry chemistry MESH: Lipopolysaccharides Bacteria |
| Zdroj: | Microbiology Microbiology, 2002, 148 (Pt 10), pp.3029-37. ⟨10.1099/00221287-148-10-3029⟩ Microbiology, Microbiology Society, 2002, 148 (Pt 10), pp.3029-37. ⟨10.1099/00221287-148-10-3029⟩ Scopus-Elsevier Europe PubMed Central |
| ISSN: | 1350-0872 1465-2080 |
| Popis: | International audience; Mycobacterium tuberculosis encodes two-component signal systems. Recently, it was established that the viability of the M. tuberculosis phoP mutant is attenuated in the mouse, suggesting the requirement of the phoP gene for M. tuberculosis intracellular growth. It is now largely acknowledged that M. tuberculosis mannosylated lipoarabinomannans (ManLAM) play a key role in M. tuberculosis intramacrophagic survival by altering the macrophage functions. So ManLAM were extracted and purified from the M. tuberculosis MT103 wild-type strain and from the M. tuberculosis phoP mutant. Their two major functional domains (i) the mannooligosaccharide caps and (ii) the mannosyl phosphatidylinositol anchor were here investigated. Using capillary electrophoresis, it is demonstrated that both mutant and wild-type M. tuberculosis strains share the same capping motifs: mono-, di- and trimannosyl alpha(1-->2) units, with the same relative abundance. Using two-dimensional NMR spectroscopy, the same acyl forms were found to be shared by both strains. However, their relative abundance was quite different. Indeed, in the phoP mutant a decrease of the triacylated ManLAM and an increase of the monoacylated ManLAM were observed. The difference in the proportion of ManLAM acyl forms and the reduced virulence of the M. tuberculosis phoP mutant are discussed. |
| Databáze: | OpenAIRE |
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