Impact of 7-Ketocholesterol and Very Long Chain Fatty Acids on Oligodendrocyte Lipid Membrane Organization: Evaluation Via LAURDAN and FAMIS Spectral Image Analysis

Autor: Aurélien Dauphin, Frédérique Frouin, Gérard Lizard, Claude-Marie Bachelet, Franck Ménétrier, Nicolas Tissot, Edmond Kahn, Alix Seguin, Zilal Kattan, Mauhamad Baarine, Kévin Ragot
Přispěvatelé: Université Pierre et Marie Curie - Paris 6 (UPMC), Université de Bourgogne (UB), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation (CSGA)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
MYELIN
law.invention
chemistry.chemical_compound
Mice
0302 clinical medicine
law
FAMIS
2-Naphthylamine
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Enzyme Inhibitors
Lipid bilayer
Ketocholesterols
0303 health sciences
Microscopy
Confocal
OXYSTEROLS
Fatty Acids
MULTIPLE-SCLEROSIS
very long chain fatty acids
Cell biology
PEROXISOMAL DISORDERS
APOPTOSIS
Oligodendroglia
X-LINKED ADRENOLEUKODYSTROPHY
medicine.anatomical_structure
Membrane
CHOLESTEROL OXIDES
lipids (amino acids
peptides
and proteins)
Laurdan
alpha-Cyclodextrins
Histology
Context (language use)
Biology
METABOLISM
Pathology and Forensic Medicine
158N oligodendrocytes
03 medical and health sciences
Membrane Lipids
Confocal microscopy
medicine
Animals
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Viability assay
Propidium iodide
LAURDAN
7-ketocholesterol
030304 developmental biology
Fluorescent Dyes
Cell Membrane
CENTRAL-NERVOUS-SYSTEM
Cell Biology
Oligodendrocyte
chemistry
CELLS
mono-photon confocal microscopy
030217 neurology & neurosurgery
Laurates
Zdroj: Cytometry Part A
Cytometry Part A, 2011, 79A (4), pp.293-305. ⟨10.1002/cyto.a.21017⟩
Cytometry Part A, Wiley, 2011, 79A (4), pp.293-305. ⟨10.1002/cyto.a.21017⟩
ISSN: 1552-4922
1552-4930
Popis: International audience; In the context of multiple sclerosis and X-linked adrenoleukodystrophy, 7-ketocholesterol (7KC) and very long chain fatty acids (C24:0, C26:0) are supposed to induce side effects respectively on oligodendrocytes which are myelin (which is a lipoproteic complex) synthesizing cells. The effects of 7KC (25, 50 mu M), C24:0 and C26:0 (10, 20 mu M) on cell viability and lipid membrane organization were investigated on 158N murine oligodendrocytes. Concerning 7KC and fatty acids (at 20 mu M only):1) cell growth was strongly inhibited; 2) marked induction of cell death was revealed with propidium iodide (PI); 3) no apoptotic cells were found with C24:0 and C26:0 (absence of cells with condensed and/or fragmented nuclei, of FLICA positive cells and of PI negative/SYTO16 negative cells); 4) some apoptotic cells were detected with 7KC. Fatty acids (at 20 mu M only) and 7KC also induced a disorganization of lipid membranes revealed with Merocyanine 540. So, to point out the effects of 7KC (25 mu M), C24:0 and C26:0 (20 mu M) on the lateral organization of lipid membranes, we used LAURDAN, which gives simultaneous information about morphology and phase state of lipid domains:its emission is blue in the ordered lipid phase, green in the disordered lipid phase. To overcome the qualitative filtering settings of blue and green emission colors, data obtained by mono- and bi-photon confocal microscopy were analyzed by spectral analysis. Sequences of emission images were obtained on both mono-and bi-photon confocal microscopes and processed by means of Factor Analysis of Medical Image Sequences (FAMIS), which is a relevant tool to unmix emission spectra and provide pure color images. Only 7KC was capable to induce a green emission with LAURDAN. Thus, at concentrations inducing oligodendrocyte cell death, 7KC (25 mu M) is more efficient than C24:0 and C26:0 (20 mu M), to trigger lateral lipid membrane disorganization. (C) 2011 International Society for Advancement of Cytometry
Databáze: OpenAIRE
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