Expression of P2X3 receptor in the trigeminal sensory nuclei of the rat
| Autor: | Juli G. Valtschanoff, Cheil Moon, Yi Sul Cho, Jin Young Bae, Yun-Sook Kim, Atsushi Yoshida, Yong Chul Bae, Se Jin Hwang, Masayuki Moritani, Dong Kuk Ahn, Ho Seob Shin, Sang Kyoo Paik |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Presynaptic Terminals Sensory system Substance P Biology Immunofluorescence Trigeminal Nuclei law.invention Rats Sprague-Dawley Trigeminal ganglion Postsynaptic potential law medicine Animals Neurons Afferent Trigeminal Nerve Microscopy Immunoelectron Cell Size Afferent Pathways Nerve Fibers Unmyelinated medicine.diagnostic_test Receptors Purinergic P2 General Neuroscience Vesicle Nociceptors Rats body regions Nociception Trigeminal Ganglion Synapses Ultrastructure Synaptic Vesicles Plant Lectins Electron microscope Neuroscience Receptors Purinergic P2X3 |
| Zdroj: | The Journal of Comparative Neurology. 506:627-639 |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.21544 |
| Popis: | Trigeminal primary afferents expressing P2X3 receptor are involved in the transmission of orofacial nociceptive information. However, little is known about their central projection pattern and ultrastructural features within the trigeminal brainstem sensory nuclei (TBSN). Here we use multiple immunofluorescence and electron microscopy to characterize the P2X3-immunopositive (+) neurons in the trigeminal ganglion and describe the distribution and synaptic organization of their central terminals within the rat TBSN, including nuclei principalis (Vp), oralis (Vo), interpolaris (Vi), and caudalis (Vc). In the trigeminal ganglion, P2X3 immunoreactivity was mainly in small and medium-sized somata, but also frequently in large somata. Although most P2X3+ somata costained for the nonpeptidergic marker IB4, few costained for the peptidergic marker substance P. Most P2X3+ fibers in the sensory root of trigeminal ganglion (92.9%) were unmyelinated, whereas the rest were small myelinated. In the TBSN, P2X3 immunoreactivity was dispersed in the rostral TBSN but was dense in the superficial laminae of Vc, especially in the inner lamina II. The P2X3+ terminals contained numerous clear, round vesicles and sparse large, dense-core vesicles. Typically, they were presynaptic to one or two dendritic shafts and also frequently postsynaptic to axonal endings, containing pleomorphic vesicles. Such P2X3+ terminals, showing glomerular shape and complex synaptic relationships, and those exhibiting axoaxonic contacts, were more frequently seen in Vp than in any other TBSN. These results suggest that orofacial nociceptive information may be transmitted via P2X3+ afferents to all TBSN and that it may be processed differently in different TBSN. J. Comp. Neurol. 506:627–639, 2008. © 2007 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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