Upconversion Nanoparticles-Based Multiplex Protein Activation to Neuron Ablation for Locomotion Regulation
| Autor: | Cuntai Zhang, Mengdie Wang, Yanxiao Ao, Wanmei Zhang, Shangbang Gao, Heng Mao, Kanyi Pu, Yan Zhang, Timothy Thatt Yang Tan, Kanghua Zeng, Fukang Qi, Louis Tao, Xiangliang Yang, Weiwei Yu, Zhongzheng Yu |
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| Rok vydání: | 2019 |
| Předmět: |
Ablation Techniques
Light Infrared Rays medicine.medical_treatment 02 engineering and technology Optogenetics 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound In vivo medicine Biological neural network Animals General Materials Science Caenorhabditis elegans Neurons Singlet Oxygen Singlet oxygen General Chemistry 021001 nanoscience & nanotechnology Ablation 0104 chemical sciences medicine.anatomical_structure chemistry Modulation Biophysics Nanoparticles Neuron 0210 nano-technology Locomotion Biotechnology Visible spectrum |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). 16(8) |
| ISSN: | 1613-6829 |
| Popis: | The optogenetic neuron ablation approach enables noninvasive remote decoding of specific neuron function within a complex living organism in high spatiotemporal resolution. However, it suffers from shallow tissue penetration of visible light with low ablation efficiency. This study reports a upconversion nanoparticle (UCNP)-based multiplex proteins activation tool to ablate deep-tissue neurons for locomotion modulation. By optimizing the dopant contents and nanoarchitecure, over 300-fold enhancement of blue (450-470 nm) and red (590-610 nm) emissions from UCNPs is achieved upon 808 nm irradiation. Such emissions simultaneously activate mini singlet oxygen generator and Chrimson, leading to boosted near infrared (NIR) light-induced neuronal ablation efficiency due to the synergism between singlet oxygen generation and intracellular Ca2+ elevation. The loss of neurons severely inhibits reverse locomotion, revealing the instructive role of neurons in controlling motor activity. The deep penetrance NIR light makes the current system feasible for in vivo deep-tissue neuron elimination. The results not only provide a rapidly adoptable platform to efficient photoablate single- and multiple-cells, but also define the neural circuits underlying behavior, with potential for development of remote therapy in diseases. |
| Databáze: | OpenAIRE |
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