Characterization of a novel thogotovirus isolated from Amblyomma testudinarium ticks in Ehime, Japan: A significant phylogenetic relationship to Bourbon virus
| Autor: | Katsunori Murota, Hiroto Shinomiya, Ken Maeda, Tetsuya Mizutani, Ryusei Kuwata, Tomomi Sato, Guillermo Posadas-Herrera, Shohei Minami, Chang Kweng Lim, Hiroko Ejiri, Kyoko Sawabe, Koki Kaku, Yukie Yamaguchi, Daisuke Kobayashi, Masayuki Saijo, Madoka Horiya, Masashi Hattori, Haruhiko Isawa, Miki Kan, Mutsuyo Takayama-Ito, Yukie Katayama, Toshiya Kimura, Hiroshi Shimoda, Ryosuke Fujita |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Virus Cultivation Ixodidae viruses Sequence Homology Tick Dhori virus Virus Replication Virus Cell Line 03 medical and health sciences Mice Viral Proteins Cytopathogenic Effect Viral Japan Orthomyxoviridae Infections Virology Animals Cluster Analysis Phylogeny Virus Release Viral matrix protein biology Amblyomma testudinarium Sequence Analysis DNA biology.organism_classification Bourbon virus Disease Models Animal 030104 developmental biology Infectious Diseases Vero cell Thogotovirus |
| Zdroj: | Virus research. 249 |
| ISSN: | 1872-7492 |
| Popis: | The genus Thogotovirus, as represented by Thogoto virus and Dhori virus, comprises a group of arthropod-borne viruses, most members of which are transmitted by ticks. Here we report the genetic and biological characterization of a new thogotovirus, designated Oz virus (OZV), isolated from the hard tick Amblyomma testudinarium in Ehime, Japan. OZV efficiently replicated and induced a cytopathic effect in Vero cells, from which enveloped pleomorphic virus particles were formed by budding. OZV could also replicate in BHK-21 and DH82 cells and caused high mortality in suckling mice after intracerebral inoculation. Phylogenetic analyses of six viral proteins indicated that OZV is clustered with Dhori and related viruses, and is most closely related in glycoprotein (GP) and matrix protein (M) sequences to Bourbon virus, a human-pathogenic thogotovirus discovered recently in the United States. Our findings emphasize the need for understanding the geographic distribution and ecology of OZV and related viruses and for reevaluation of the medical and public health importance of thogotoviruses. |
| Databáze: | OpenAIRE |
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