The protective effect of vitamin D against carbon tetrachloride damage to the rat liver

Autor:	Kazime Gonca Akbulut, Şevin Güney, Güler Öztürk, Nesrin Özsoy, Dilşad Özerkan
Rok vydání:	2017
Předmět:	Male 0301 basic medicine medicine.medical_specialty Histology Antioxidant medicine.medical_treatment medicine.disease_cause Polymerase Chain Reaction digestive system 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Vitamin D and neurology Animals Rats Wistar Vitamin D Carbon Tetrachloride biology Albumin Reverse Transcription General Medicine Reference Standards Immunohistochemistry digestive system diseases Rats Medical Laboratory Technology 030104 developmental biology Endocrinology Liver chemistry Alanine transaminase Carbon tetrachloride biology.protein Alkaline phosphatase 030211 gastroenterology & hepatology Hepatic fibrosis Oxidative stress
Zdroj:	Biotechnic & Histochemistry. 92:513-523
ISSN:	1473-7760 1052-0295
DOI:	10.1080/10520295.2017.1361549
Popis:	We investigated the protective effect of vitamin D against liver damage caused by carbon tetrachloride (CCl4). Twenty-four male rats were divided into four equal groups: G1, untreated controls; G2, administered CCl4; G3, administered both CCl4 and vitamin D for 10 weeks; G4, administered CCl4 for 10 weeks and vitamin D for 12 weeks. At the end of experiment, intracardiac blood samples were taken and liver samples were removed. Hepatic damage due to CCl4 was assessed using biochemistry and histopathology. Glutathione (GSH) levels decreased, while malondialdehyde (MDA) levels increased in liver tissues of G2. Alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl-transaminase (GGT) levels increased, while albumin (ALB) levels decreased. Hepatocyte degeneration, lobular disorder, sinusoid dilation, focal necrotic areas, hyperemia, and glycogen loss were observed. Hepatic fibrosis was observed around portal areas and central veins. Bridging fibrous septa were formed between portal veins. By immunohistochemistry, both matrix metalloproteinase-9 (MMP-9) and desmin reactivity were increased. All aspects of liver damage were at least partially prevented in rats treated with vitamin D. Vitamin D appears to act as an antioxidant and anti-fibrotic to protect the rat liver against damage.
Databáze:	OpenAIRE
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