Ca2+ signaling in gerbil CA3 hippocampal neurons following transient in vivo ischemia
| Autor: | John A. Connor, C.W.R. Shuttleworth, A.C. Greenwood |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Time Factors Fura-2 Central nervous system Ischemia Hippocampus Action Potentials Hippocampal formation In Vitro Techniques Gerbil chemistry.chemical_compound medicine Animals Calcium Signaling Cell Death business.industry General Neuroscience Pyramidal Cells medicine.disease Electric Stimulation Cell biology Electrophysiology medicine.anatomical_structure nervous system chemistry Reperfusion Injury Calcium Neuron death business Gerbillinae Neuroscience Signal Transduction |
| Zdroj: | Neuroscience letters. 286(2) |
| ISSN: | 0304-3940 |
| Popis: | Using the gerbil model of post-ischemic neuron death in the hippocampal CA1 region, it was recently shown that there is a strong down-regulation of voltage-gated Ca 2+ influx in neurons examined at 2 days after the ischemic insult (Connor, J.A., Razani-Boroujerdi, S., Greenwood, A.C., Cormier, R.J., Petrozzino, J.J. and Lin, R.C., Reduced voltage-dependent Ca 2+ signaling in CA1 neurons after brief ischemia in gerbils, J. Neurophysiol., 81 (1999) 299–306). The aim of the present study was to determine whether a similar change occurs in pyramidal neurons of the CA3 region that are relatively resistant to transient ischemia. In vitro intracellular recordings and fluorometric Ca 2+ measurements were made from CA3 neurons in coronal slices prepared from controls and 1 or 2 days following in vivo ischemia. In slices from control and post-ischemic animals, the electrophysiological properties of CA3 neurons were consistent with significant voltage-gated Ca 2+ influx, leading to spike frequency adaptation. Quantitative results indicated no significant difference in Ca 2+ transients evoked by action potential trains. This Ca 2+ signaling was compared with responses in CA1 neurons from the same preparations, which showed substantially diminished Ca 2+ influx at 2 days post-ischemia. These findings suggest that diminished Ca 2+ -signaling is not a general feature of pyramidal neurons following ischemia, but is characteristic of neurons destined to die. |
| Databáze: | OpenAIRE |
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