Effects of a Small Interfering RNA Targeting YKL-40 Gene on the Proliferation and Invasion of Endometrial Cancer HEC-1A Cells
| Autor: | Da-hai Li, Jiang-tao Fan, Yan Liu, Lili Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Small interfering RNA Biology law.invention 03 medical and health sciences 0302 clinical medicine Cell Movement law Cell Line Tumor medicine Humans Chitinase-3-Like Protein 1 RNA Small Interfering Gene Polymerase chain reaction Cell Proliferation Cell growth Endometrial cancer Obstetrics and Gynecology Transfection medicine.disease Small molecule Endometrial Neoplasms Cell biology 030104 developmental biology Oncology 030220 oncology & carcinogenesis Female Drug Screening Assays Antitumor Cell Migration Assays |
| Zdroj: | International Journal of Gynecologic Cancer. 26:1190-1195 |
| ISSN: | 1525-1438 1048-891X |
| DOI: | 10.1097/igc.0000000000000774 |
| Popis: | ObjectiveThe purpose of this study was to explore the effects of a small interfering RNA (siRNA) targeting YKL-40 on the proliferation and invasion of endometrial cancer (EC) HEC-1A cells.MethodsWe used an siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40. MTT, migration, and invasion assays were performed to identify the effects of si-YKL-40 on the proliferation, migration, and invasive abilities of the HEC-1A cells.ResultsmRNA expression of YKL-40 was down-regulated in HEC-1A cells after transfection with si-YKL-40 (P < 0.05). The proliferation, migration, and invasive abilities of HEC-1A cells were inhibited by siRNA (P < 0.05).ConclusionsYKL-40 targeting siRNA specifically blocks the activity of YKL-40 in human EC HEC-1A cells, resulting in tumor suppression. This indicates that YKL-40 might serve as a potential small molecule target in the treatment of EC. |
| Databáze: | OpenAIRE |
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