Association mapping of seed and disease resistance traits in Theobroma cacao L

Autor: Pathmanathan Umaharan, Lyndel W. Meinhardt, Lambert A. Motilal, Dapeng Zhang, Claire Lanaud, Sue Mischke, Olivier Fouet, Michel Boccara
Rok vydání: 2016
Předmět:
Graine
0106 biological sciences
0301 basic medicine
Résistance génétique
Linkage disequilibrium
Theobroma
Polymorphisme génétique
Phénotype
Moniliophthora
Plant Science
01 natural sciences
Linkage Disequilibrium
F30 - Génétique et amélioration des plantes
Génétique des populations
Plant Immunity
Marqueur génétique
Association mapping
Genetics
biology
Chromosome Mapping
Phytophthora palmivora
Seeds
Microsatellite
Génotype
Genetic Markers
Locus des caractères quantitatifs
Single-nucleotide polymorphism
Plant disease resistance
Polymorphism
Single Nucleotide
Équilibre génétique
03 medical and health sciences
Quantitative Trait
Heritable
Theobroma cacao
H20 - Maladies des plantes
Cacao
Phenotypic trait
Résistance aux maladies
biology.organism_classification
Amélioration des plantes
Plant Breeding
030104 developmental biology
Carte génétique
Genome-Wide Association Study
Microsatellite Repeats
010606 plant biology & botany
Zdroj: Planta
ISSN: 1432-2048
0032-0935
Popis: Microsatellite and single nucleotide polymorphism markers that could be used in marker assisted breeding of cacao were identified for number of filled seeds, black pod resistance and witches’ broom disease resistance. An association mapping approach was employed to identify markers for seed number and resistance to black pod and witches’ broom disease (WBD) in cacao (Theobroma cacao L.). Ninety-five microsatellites (SSRs) and 775 single nucleotide polymorphisms (SNPs) were assessed on 483 unique trees in the International Cocoa Genebank Trinidad (ICGT). Linkage disequilibrium (LD) and association mapping studies were conducted to identify markers to tag the phenotypic traits. Decay of LD occurred over an average 9.3 cM for chromosomes 1–9 and 2.5 cM for chromosome 10. Marker/trait associations were generally identified based on general linear models (GLMs) that incorporated principal components from molecular information on relatedness factor. Seven markers (mTcCIR 8, 66, 126, 212; TcSNP368, 697, 1370) on chromosomes 1 and 9 were identified for number of filled seeds (NSEED). A single marker was found for black pod resistance (mTcCIR280) on chromosome 3, whereas six markers on chromosomes 4, 5, 6, 8, and 10 were detected for WBD (mTcCIR91, 183; TcSNP375, 720, 1230 and 1374). It is expected that this association mapping study in cacao would contribute to the knowledge of the genetic determinism of cocoa traits and that the markers identified herein would prove useful in marker assisted breeding of cacao.
Databáze: OpenAIRE
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