Morphological and biomechanical effects of annulus fibrosus injury and repair in an ovine cervical model
Autor: | Sibylle Grad, Zhen Li, Abhay Pandit, Rose G. Long, Mauro Alini, Daisuke Sakai, Tanja Schmid, David Eglin, Theodor Di Pauli von Treuheim, Lorin Michael Benneker, Stephan Zeiter, Dirk W. Grijpma, Stephen J. Ferguson, Ursula Eberli, James C. Iatridis, Dirk Nehrbass |
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Přispěvatelé: | TechMed Centre, Biomaterials Science and Technology |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Pathology
medicine.medical_specialty ovine in vivo model medicine.medical_treatment 0206 medical engineering 610 Medicine & health 02 engineering and technology Degeneration (medical) Fibrin 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery Tissue engineering medicine annulus fibrosus Orthopedics and Sports Medicine Research Articles Reduction (orthopedic surgery) biology business.industry Biomaterial intervertebral disc intervertebral disc herniation tissue engineering Intervertebral disc 020601 biomedical engineering 3. Good health Resorption lcsh:RD701-811 medicine.anatomical_structure biology.protein business 030217 neurology & neurosurgery Research Article Blood vessel |
Zdroj: | JOR Spine, 3(1):e1074. Wiley-Blackwell JOR Spine, Vol 3, Iss 1, Pp n/a-n/a (2020) JOR Spine, 3 (1) Long, Rose G; Ferguson, Stephen J; Benneker, Lorin M.; Sakai, Daisuke; Li, Zhen; Pandit, Abhay; Grijpma, Dirk W; Eglin, David; Zeiter, Stephan; Schmid, Tanja; Eberli, Ursula; Nehrbass, Dirk; Di Pauli von Treuheim, Theodor; Alini, Mauro; Iatridis, James C; Grad, Sibylle (2020). Morphological and biomechanical effects of annulus fibrosus injury and repair in an ovine cervical model. JOR Spine, 3(1), e1074. Wiley 10.1002/jsp2.1074 JOR Spine |
ISSN: | 2572-1143 |
Popis: | Tissue engineering repair of annulus fibrosus (AF) defects has the potential to prevent disability and pain from intervertebral disc (IVD) herniation and its progression to degeneration. Clinical translation of AF repair methods requires assessment in long‐term large animal models. An ovine AF injury model was developed using cervical spinal levels and a biopsy‐type AF defect to assess composite tissue engineering repair in 1‐month and 12‐month studies. The repair used a fibrin hydrogel crosslinked with genipin (FibGen) to seal defects, poly(trimethylene carbonate) (PTMC) scaffolds to replace lost AF tissue, and polyurethane membranes to prevent herniation. In the 1‐month study, PTMC scaffolds sealed with FibGen herniated with polyurethane membranes. When applied alone, FibGen integrated with the surrounding AF tissue without herniation, showing promise for long‐term studies. The 12‐month long‐term study used only FibGen which showed fibrous healing, biomaterial resorption and no obvious hydrogel‐related complications. However, the 2 mm biopsy punch injury condition also exhibited fibrotic healing at 12 months. Both untreated and FibGen treated groups showed equivalency with no detectable differences in histological grades of proteoglycans, cellular morphology, IVD structure and blood vessel formation, biomechanical properties including torque range and axial range of motion, Pfirrmann grade, IVD height, and quantitative scores of vertebral body changes from clinical computed tomography. The biopsy‐type injury caused endplate defects with a high prevalence of osteophytes in all groups and no nucleus herniation, indicating that the biopsy‐type injury requires further refinement, such as reduction to a slit‐type defect that could penetrate the full depth of the AF without damaging the endplate. Results demonstrate translational feasibility of FibGen for AF repair to seal AF defects, although future study with a more refined injury model is required to validate the efficacy of FibGen before translation. ISSN:2572-1143 |
Databáze: | OpenAIRE |
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