Macro to microfluidics system for biological environmental monitoring
| Autor: | Christine Peponnet, Cédric Allier, Yves Fouillet, Arnaud Rival, Frederic Bottausci, Denis Bouvier, Dorothée Jary, Guillaume Delapierre, Manuelle Quinaud, Cyril Delattre, Laurent Davoust |
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| Přispěvatelé: | Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Science et Ingénierie des Matériaux et Procédés (SIMaP), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
Computer science
Microfluidics Biomedical Engineering Biophysics Nanotechnology 02 engineering and technology 01 natural sciences Polymerase Chain Reaction Sensitivity and Specificity Electrochemistry Escherichia coli Humans Fluidics Digital microfluidics Protocol (object-oriented programming) Throughput (business) business.industry Adenoviruses Human 010401 analytical chemistry General Medicine [CHIM.MATE]Chemical Sciences/Material chemistry DNA Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Automation 0104 chemical sciences Streptococcus pneumoniae Electrowetting Sample collection 0210 nano-technology business Baculoviridae Computer hardware Biotechnology Bacillus subtilis Environmental Monitoring |
| Zdroj: | Biosensors & bioelectronics Biosensors & bioelectronics, 2012, 36 (1), pp.230-235. ⟨10.1016/j.bios.2012.04.024⟩ |
| ISSN: | 1873-4235 |
| DOI: | 10.1016/j.bios.2012.04.024⟩ |
| Popis: | International audience; Biological environmental monitoring (BEM) is a growing field of research which challenges both microfluidics and system automation. The aim is to develop a transportable system with analysis throughput which satisfies the requirements: (i) fully autonomous, (ii) complete protocol integration from sample collection to final analysis, (iii) detection of diluted molecules or biological species in a large real life environmental sample volume, (iv) robustness and (v) flexibility and versatility. This paper discusses all these specifications in order to define an original fluidic architecture based on three connected modules, a sampling module, a sample preparation module and a detection module. The sample preparation module highly concentrates on the pathogens present in a few mL samples of complex and unknown solutions and purifies the pathogens' nucleic acids into a few mu L of a controlled buffer. To do so. a two-step concentration protocol based on magnetic beads is automated in a reusable macro-to-micro fluidic system. The detection module is a PCR based miniaturized platform using digital microfluidics, where reactions are performed in 64 nL droplets handled by electrowetting on dielectric (EWOD) actuation. The design and manufacture of the two modules are reported as well as their respective performances. To demonstrate the integration of the complete protocol in the same system, first results of pathogen detection are shown. |
| Databáze: | OpenAIRE |
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