A command-like descending neuron that coordinately activates backward and inhibits forward locomotion
| Autor: | Chris Q. Doe, Arnaldo Carreira-Rosario, Laurina Manning, Alberto Cardona, Aref Arzan Zarin, Matthew Q Clark, Richard D. Fetter |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Backward walking Backward locomotion fungi Reciprocal inhibition Biology Optogenetics Forward locomotion 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure medicine Biological neural network Neuron Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/339556 |
| Popis: | Command-like descending neurons can induce many behaviors, such as backward locomotion, escape, feeding, courtship, egg-laying, or grooming. In most animals it remains unknown how neural circuits switch between these antagonistic behaviors: via top-down activation/inhibition of antagonistic circuits or via reciprocal inhibition between antagonistic circuits. Here we use genetic screens, intersectional genetics, circuit reconstruction by electron microscopy, and functional optogenetics to identify a bilateral pair of larval “mooncrawler descending neurons” (MDNs) with command-like ability to coordinately induce backward locomotion and block forward locomotion; the former by activating a backward-specific premotor neuron, and the latter by disynaptic inhibition of a forward-specific premotor neuron. In contrast, direct reciprocal inhibition between forward and backward circuits was not observed. Thus, MDNs coordinate a transition between antagonistic larval locomotor behaviors. Interestingly, larval MDNs persist into adulthood, where they can trigger backward walking. Thus, MDNs induce backward locomotion in both limbless and limbed animals.HighlightsMDN command-like descending neuron induces backward larval locomotionMDN neurons coordinately regulate antagonistic behaviors (forward/backward locomotion)MDN-motor circuit validated at structural (TEM) and functional (optogenetic) levelsMDN neurons induce backward locomotion in both limbless larva and limbed adult |
| Databáze: | OpenAIRE |
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