Encapsulation of glucose oxidase in Fe(III)/tannic acid nanocomposites for effective tumor ablation via Fenton reaction
| Autor: | Mei Liu, Zhifei Wang, Nongyue He, Rongmu Lv, Qianqian Liu, Keke Du |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
Materials science Cell Survival Drug Compounding Radical Breast Neoplasms Bioengineering 02 engineering and technology 010402 general chemistry Ferric Compounds 01 natural sciences Nanocomposites law.invention Catalysis Glucose Oxidase chemistry.chemical_compound Magazine In vivo law Tannic acid Humans General Materials Science Glucose oxidase Electrical and Electronic Engineering Cell Proliferation chemistry.chemical_classification Reactive oxygen species Nanocomposite biology Hydroxyl Radical Mechanical Engineering Hydrogen Peroxide General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Mechanics of Materials MCF-7 Cells biology.protein Female Reactive Oxygen Species 0210 nano-technology Tannins Nuclear chemistry |
| Zdroj: | Nanotechnology. 31:015101 |
| ISSN: | 1361-6528 0957-4484 |
| DOI: | 10.1088/1361-6528/ab44f9 |
| Popis: | Increasing the content of reactive oxygen species (ROS) with the assistance of nanoformulations in cancer cells via the Fenton reaction is considered an effective method to treat cancer. However, the efficiency of the Fenton reaction is affected by the level of H2O2, the selection of iron ions in different nanoformulations, etc. Herein, we use FeIII-tannic acid (FeIIITA) nanocomposites as the carrier to deliver glucose oxidase (GOD) which can solve the problem of insufficient endogenous H2O2 by catalytically converting the glucose. In comparison with traditional Fe2+/Fe3+, FeIIITA nanocomposites perform higher catalytic activity in converting H2O2 to high toxic hydroxyl radicals (·OH) due to the TA-mediated reduction of Fe3+. So, the integration of GOD and TA in the construction of nanocomposites significantly enhances the efficiency of the Fenton reaction. In vitro experiments show that ·OH produced by GOD-FeIIITA nanocomposites can not only achieve a good anticancer effect at a low concentration but also promote degradability of the nanocomposites. When it is only 1.08 μg · ml-1, the cell apoptosis rate has reached 76.91%. In vivo experiments further demonstrate that GOD-FeIIITA nanocomposites can significantly inhibit tumor growth. So this work lays a good foundation for Fenton reaction-based cancer treatment. |
| Databáze: | OpenAIRE |
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