Vascular smooth muscle cell differentiation from human stem/progenitor cells
| Autor: | Mansoor Husain, Sarah K. Steinbach |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Human Embryonic Stem Cells Induced Pluripotent Stem Cells Myocytes Smooth Muscle Cell Culture Techniques Biology Regenerative medicine Culture Media Serum-Free Muscle Smooth Vascular General Biochemistry Genetics and Molecular Biology Cell therapy 03 medical and health sciences Animals Humans Progenitor cell Induced pluripotent stem cell Molecular Biology Cells Cultured Cell Differentiation musculoskeletal system Embryonic stem cell 3. Good health Cell biology Endothelial stem cell 030104 developmental biology Vascular smooth muscle cell differentiation Immunology cardiovascular system tissues |
| Zdroj: | Methods. 101:85-92 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2015.12.004 |
| Popis: | Transplantation of vascular smooth muscle cells (VSMCs) is a promising cellular therapy to promote angiogenesis and wound healing. However, VSMCs are derived from diverse embryonic sources which may influence their role in the development of vascular disease and in its therapeutic modulation. Despite progress in understanding the mechanisms of VSMC differentiation, there remains a shortage of robust methods for generating lineage-specific VSMCs from pluripotent and adult stem/progenitor cells in serum-free conditions. Here we describe a method for differentiating pluripotent stem cells, such as embryonic and induced pluripotent stem cells, as well as skin-derived precursors, into lateral plate-derived VSMCs including 'coronary-like' VSMCs and neural crest-derived VSMC, respectively. We believe this approach will have broad applications in modeling origin-specific disease vulnerability and in developing personalized cell-based vascular grafts for regenerative medicine. |
| Databáze: | OpenAIRE |
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