Transplantation of neural stem cells in the mouse model of ischemic brain stroke and expression of genes involved in programmed cell death
| Autor: | Arijana Đemaili, Iva Salamon, Valentina Hribljan, Ivan Alić, Dinko Mitrečić |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Programmed cell death Ubiquitin-Protein Ligases Necroptosis Cellular differentiation Apoptosis Mice Transgenic Caspase 3 MCAO stroke model Stem cell transplantation programmed cell death Biology Brain Ischemia Mice 03 medical and health sciences RIPK1 Neural Stem Cells Basic Science Animals RNA Messenger Protein kinase A Reverse Transcriptase Polymerase Chain Reaction Apoptosis Inducing Factor Cell Differentiation General Medicine Neural stem cell Cell biology Mice Inbred C57BL Stroke Transplantation Disease Models Animal 030104 developmental biology Gene Expression Regulation Caspases Receptor-Interacting Protein Serine-Threonine Kinases Female Cyclophilin A Protein Kinases Stem Cell Transplantation |
| Zdroj: | Croatian Medical Journal Volume 59 Issue 5 |
| ISSN: | 0353-9504 1332-8166 |
| Popis: | Aim: To analyze how neural stem cells (NSC) transplantation in the stroke-affected mouse brain influences the expression of genes involved in apoptosis-inducing factor (AIF)-mediated cell death – apoptosis inducing factor mitochondria associated 1 (Aifm1), ring finger protein 146 (Rnf146, Iduna), and cyclophilin A (CypA); necroptosis –receptor interaction protein kinase 1 (Ripk1), Ripk3, and mixed-lineage kinase domain-like protein (Mlkl); and apoptosis – Caspase 3 (Casp3) and Casp8. Methods: Four groups of animals were used to obtain mRNA for quantitative reverse transcription polymerase chain reaction analysis: healthy animals (n = 3), animals with stroke (n = 4), animals with stroke treated by stem cell transplantation (n = 7), and animals with stroke treated by proliferation-supporting medium (n = 5). Ischemic brain injury was induced by transient left middle cerebral artery occlusion. Statistical analysis was performed using one-way analysis of variance with post-hoc Tukey test. Results: NSC transplantation in the stroke-affected mouse brain significantly increased the expression of Iduna (P |
| Databáze: | OpenAIRE |
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