Sclerostin Antibody Treatment Enhances Rotator Cuff Tendon-to-Bone Healing in an Animal Model
| Autor: | Stavros Thomopoulos, Shivam A. Shah, Michael S. Ominsky, Necat Havlioglu, Ioannis Kormpakis, Leesa M. Galatz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetic Markers medicine.medical_specialty Scientific Articles Bone density Injections Subcutaneous Osteoporosis Bone healing Antibodies Bone and Bones Rotator Cuff Injuries Tendons 03 medical and health sciences chemistry.chemical_compound Rotator Cuff 0302 clinical medicine Bone Density Osteogenesis Tendon Injuries medicine Animals Orthopedics and Sports Medicine Rotator cuff Bone Resorption Adaptor Proteins Signal Transducing Bone mineral 030222 orthopedics Wound Healing business.industry General Medicine medicine.disease Surgery Tendon Biomechanical Phenomena Disease Models Animal 030104 developmental biology medicine.anatomical_structure Treatment Outcome chemistry Cuff Bone Morphogenetic Proteins Models Animal Humeral Head Sclerostin business |
| Popis: | Background Rotator cuff tears are a common source of pain and disability, and poor healing after repair leads to high retear rates. Bone loss in the humeral head before and after repair has been associated with poor healing. The purpose of the current study was to mitigate bone loss near the repaired cuff and improve healing outcomes. Methods Sclerostin antibody (Scl-Ab) treatment, previously shown to increase bone formation and strength in the setting of osteoporosis, was used in the current study to address bone loss and enhance rotator cuff healing in an animal model. Scl-Ab was administered subcutaneously at the time of rotator cuff repair and every 2 weeks until the animals were sacrificed. The effect of Scl-Ab treatment was evaluated after 2, 4, and 8 weeks of healing, using bone morphometric analysis, biomechanical evaluation, histological analysis, and gene expression outcomes. Results Injury and repair led to a reduction in bone mineral density after 2 and 4 weeks of healing in the control and Scl-Ab treatment groups. After 8 weeks of healing, animals receiving Scl-Ab treatment had 30% greater bone mineral density than the controls. A decrease in biomechanical properties was observed in both groups after 4 weeks of healing compared with healthy tendon-to-bone attachments. After 8 weeks of healing, Scl-Ab-treated animals had improved strength (38%) and stiffness (43%) compared with control animals. Histological assessment showed that Scl-Ab promoted better integration of tendon and bone by 8 weeks of healing. Scl-Ab had significant effects on gene expression in bone, indicative of enhanced bone formation, and no effect on the expression of genes in tendon. Conclusions This study provides evidence that Scl-Ab treatment improves tendon-to-bone healing at the rotator cuff by increasing attachment-site bone mineral density, leading to improved biomechanical properties. Clinical relevance Scl-Ab treatment may improve outcomes after rotator cuff repair. |
| Databáze: | OpenAIRE |
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