Detecção do Southern bean mosaic virus no Paraná, e separação do Bean rugose mosaic virus em feijoeiro

Autor:	Eliezer Rodrigues de Souto, Anésio Bianchini, Álvaro M. R. Almeida, José O. Gaspar, Elliot W. Kitajima, Marcos Diamantino G. Gasparin
Přispěvatelé:	Universidade Estadual de Maringá (UEM), Instituto Agronômico do Paraná, Universidade Estadual Paulista (Unesp), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Universidade de São Paulo (USP)
Rok vydání:	2005
Předmět:	BRMV Plant virus SBMV Coat Proteins Plant Science Biology biology.organism_classification Sobemovirus Molecular biology
Zdroj:	SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP
ISSN:	0100-4158
DOI:	10.1590/s0100-41582005000100013
Popis:	Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:02Z No. of bitstreams: 1 S0100-41582005000100013.pdf: 985300 bytes, checksum: 29c02fbdb0e8a897f8867d8604cee182 (MD5) Made available in DSpace on 2013-08-22T18:49:02Z (GMT). No. of bitstreams: 1 S0100-41582005000100013.pdf: 985300 bytes, checksum: 29c02fbdb0e8a897f8867d8604cee182 (MD5) Previous issue date: 2005-02-01 Made available in DSpace on 2013-09-30T19:39:11Z (GMT). No. of bitstreams: 2 S0100-41582005000100013.pdf: 985300 bytes, checksum: 29c02fbdb0e8a897f8867d8604cee182 (MD5) S0100-41582005000100013.pdf.txt: 21059 bytes, checksum: 08dc15a218b45c60aa4941f47c46dcf0 (MD5) Previous issue date: 2005-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:03:52Z No. of bitstreams: 2 S0100-41582005000100013.pdf: 985300 bytes, checksum: 29c02fbdb0e8a897f8867d8604cee182 (MD5) S0100-41582005000100013.pdf.txt: 21059 bytes, checksum: 08dc15a218b45c60aa4941f47c46dcf0 (MD5) Made available in DSpace on 2014-05-20T14:03:52Z (GMT). No. of bitstreams: 2 S0100-41582005000100013.pdf: 985300 bytes, checksum: 29c02fbdb0e8a897f8867d8604cee182 (MD5) S0100-41582005000100013.pdf.txt: 21059 bytes, checksum: 08dc15a218b45c60aa4941f47c46dcf0 (MD5) Previous issue date: 2005-02-01 Em lavouras de feijoeiro (Phaseolus vulgaris) da cultivar Carioca Comum, no município de Londrina, Estado do Paraná, foram encontradas plantas com sintomas de necrose da haste, mosaico clorótico leve e porte reduzido, semelhantes aos sintomas causados por infecção viral. Exames de microscopia eletrônica revelaram a presença de partículas isométricas. em testes de imunodifusão dupla em gel de ágar os extratos foliares de plantas infetadas reagiram positivamente com anti-soro específico para o Southern bean mosaic virus (SBMV). O vírus foi purificado e a massa molecular de sua proteína capsidial foi estimada em 30 kDa, valor esperado para proteínas do capsídeo de vírus do gênero Sobemovirus. A gama de hospedeiras do SBMV isolado no Paraná foi restrita ao feijoeiro e a algumas cultivares de soja (Glycine max). A separação de dois vírus isométricos comuns em infecções mistas no feijoeiro foi possível através da reação de imunidade ao SBMV apresentada por Crotalaria sp, Chenopodium quinoa e Mucuna deeringiana, e da reação de susceptibilidade dessas mesmas hospedeiras ao Bean rugose mosaic virus (BRMV). Plants of bean (Phaseolus vulgaris), showing symptoms of stunt, stem necrosis and chlorotic mosaic, similar to those induced by virus infection were found in a bean field in Londrina, Paraná. Electron microscopy examinations showed isometric virus particles in the cell cytoplasm. Double immunodifusion serological tests with antiserum for Southern bean mosaic virus (SBMV) gave positive results when tested against plant sap from infected bean plants. The virus was purified and the molecular mass of its coat protein was estimated as 30 kDa, the expected value for the coat protein of viruses from the genus Sobemovirus. The host range of the virus was restricted to bean and some soybean (Glycine max) cultivars. It was possible to separate two isometric viruses commonly found in bean based on the immunity reaction of Crotalaria sp., Chenopodium quinoa and Mucuna deeringiana to SBMV, and on the susceptibility reaction of the same hosts to Bean rugose mosaic virus (BRMV). Universidade Estadual de Maringá (UEM) CCA DAG Instituto Agronômico do Paraná UNESP IBILC Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Soja USP ESALQ UNESP IBILC
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3cd184a4d4cd51b008a5d8de95bd1c6 https://doi.org/10.1590/s0100-41582005000100013 Zobrazit plný text záznamu