Age-related decline in hypocretin (orexin) receptor 2 messenger RNA levels in the mouse brain
| Autor: | Thomas S. Kilduff, Akira Terao, Stephen R. Morairty, A. Apte-Deshpande, Yvonne R. Freund |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Receptors Neuropeptide medicine.medical_specialty Aging Central nervous system Hippocampus Dynorphin Biology Receptors G-Protein-Coupled Hypocretin (orexin) receptor 2 Mice Orexin Receptors Internal medicine medicine Animals RNA Messenger Protein Precursors Brain Chemistry Basal forebrain General Neuroscience Brain Enkephalins Orexin receptor Orexin Mice Inbred C57BL Endocrinology medicine.anatomical_structure nervous system Gene Expression Regulation Hypothalamus |
| Zdroj: | Neuroscience letters. 332(3) |
| ISSN: | 0304-3940 |
| Popis: | The hypocretin (Hcrt; also known as orexin) system has been implicated in arousal state regulation and energy metabolism. We hypothesize that age-related sleep problems can result from dysfunction of this system and thus measured messenger RNA (mRNA) levels of preprohcrt in the hypothalamus, and hcrt receptor 1 (hcrtr1) and hcrt receptor 2 (hcrtr2) in eight brain regions of 3, 12, 18 and 24 months old C57BL/6 mice. Expression of preprohcrt and the colocalized prodynorphin did not change with age. Whereas an age-related change in hcrtr1 mRNA expression was observed only in the hippocampus, hcrtr2 mRNA levels declined in the hippocampus, thalamus, pons, and medulla; these reductions ranged from 33 to 44%. Declining trends (P < 0.1) in hcrtr2 mRNA levels were also observed in the cortex, basal forebrain and hypothalamus. These results are consistent with the hypothesis that an age-related deterioration occurs in the Hcrt system that may contribute to age-related sleep disorders. |
| Databáze: | OpenAIRE |
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