Urine metabolomics of women from small villages exposed to high environmental cadmium levels

Autor: Jin Wang, Liuying Tang, Chunsui Liang, Yanhong Gao, Wencai Chen, Gangfeng Ouyang, Qiong Huang, Xing-fen Yang, Yinghua Xu, Xuxia Liang
Rok vydání: 2016
Předmět:
Zdroj: Environmental Toxicology and Chemistry. 35:1268-1275
ISSN: 1552-8618
0730-7268
DOI: 10.1002/etc.3274
Popis: The present study aimed to identify urine metabolites in women exposed to high cadmium (Cd) levels. Twenty-one women exposed to environmental Cd and 12 age-matched controls were categorized as high exposure (urine Cd ≥ 15 μg/g creatinine; n = 9) or low exposure (15 μg/g creatinine > urine Cd > 5 μg/g creatinine; n = 12). Low-molecular weight metabolites in urine were analyzed by gas chromatography and mass spectrometry after derivatization. An orthogonal partial least-squares discriminant analysis model was constructed, and metabolites from the dimensional model were selected according to the variable importance in projection (>1). Metabolites differing significantly in abundance between different exposure groups were identified by searching mass spectral databases, and related pathways were analyzed using the Kyoto Encyclopedia of Genes and Genomes. Approximately 110 significantly different metabolites were detected with variable importance in projection > 1, and 48 of them were found to differ markedly in abundance among the 3 groups. Twenty-seven matched with known metabolites, including 22 significantly increased and 5 markedly decreased in the high-exposure group (p
Databáze: OpenAIRE
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