Ethanol Production from Sugarcane Bagasse by Means of Enzymes Produced by Solid State Fermentation Method

Autor: Shaibani, Nasim, Ghazvini, Saba, Andalibi, Mohammad R., Yaghmaei, Soheila
Jazyk: angličtina
Rok vydání: 2011
Předmět:
DOI: 10.5281/zenodo.1082667
Popis: Nowadays there is a growing interest in biofuel production in most countries because of the increasing concerns about hydrocarbon fuel shortage and global climate changes, also for enhancing agricultural economy and producing local needs for transportation fuel. Ethanol can be produced from biomass by the hydrolysis and sugar fermentation processes. In this study ethanol was produced without using expensive commercial enzymes from sugarcane bagasse. Alkali pretreatment was used to prepare biomass before enzymatic hydrolysis. The comparison between NaOH, KOH and Ca(OH)2 shows NaOH is more effective on bagasse. The required enzymes for biomass hydrolysis were produced from sugarcane solid state fermentation via two fungi: Trichoderma longibrachiatum and Aspergillus niger. The results show that the produced enzyme solution via A. niger has functioned better than T. longibrachiatum. Ethanol was produced by simultaneous saccharification and fermentation (SSF) with crude enzyme solution from T. longibrachiatum and Saccharomyces cerevisiae yeast. To evaluate this procedure, SSF of pretreated bagasse was also done using Celluclast 1.5L by Novozymes. The yield of ethanol production by commercial enzyme and produced enzyme solution via T. longibrachiatum was 81% and 50% respectively.
{"references":["A. Demirbas, \"Biofuels sources, biofuel policy, biofuel economy and\nglobal biofuel projections\", Energy Convers. Manage., vol. 49, pp.\n2106-2116, 2008.","A. da Silva, H. Inoue, T. Endo, Sh. Yano, E. Bon, \"Milling pretreatment\nof sugarcane bagasse and straw for enzymatic hydrolysis and ethanol\nfermentation\", Bioresour. Technol., vol. 101, pp. 7402-7409, 2010.","J. Seabra, L. Tao, H. Chum, I. Macedo, \"A techno-economic evaluation\nof the effects of centralized cellulosic ethanol and co-products refinery\noptions with sugarcane mill clustering\", Biomass Bioenerg., vol. 34,\npp.1065-78, 2010.","A. Edgardo, P. Carolina, R. Manuel, F. Juanita, B. Jaime, \"Selection of\nthermotolerant yeast strains Saccharomyces cerevisiae for bioethanol\nproduction\", Enzyme Microb. Technol., vol. 43, pp. 120-123, 2008.","HZ. Chen, WH. Qiu, \"Key technologies for bioethanol production from\nlignocelluloses\", Biotechnol. Adv., vol. 28, pp. 556-562, 2010.","U. Hölker, M. Höfer, J. Lenz, \"Biotechnological advantages of\nlaboratory-scale solid-state fermentation with fungi\", Appl. Micrbiol.\nBiotechnol. Vol. 64, pp. 175-186, 2004.","M. Lever, G. Ho, R. Cord-Ruwisch, \"Ethanol from lignocellulose using\ncrude unprocessed cellulose from solid-state fermentation\", Bioresour.\nTechnol., vol. 101, pp. 7083-7087, 2010.","R. Sukumaran, R. Singhania, G. Mathew, A. Pandey, \"Cellulase\nproduction using biomass feed stock and its application in\nlignocellulose saccharification for bio-ethanol production\", Renew.\nEnerg., vol. 34, pp. 421-4, 2009.","H. J├©rgensen, J. B. Kristensen, C. Felby, \"Enzymatic conversion of\nlignocellulose into fermentable sugars: challenges and opportunities\",\nBiofuels Bioprod Bioref., vol. 1, pp. 119-134, 2007.\n[10] A. Sluiter, B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton,\n\"Determination of structural carbohydrates and lignin in biomass.\nNational renewable energy laboratory\", Technical Report, NREL/TP-\n510-42618, Golden, Colorado, April 2008.\n[11] T. K. Ghose, \"Measurement of cellulase activities\", Pure & Appl.\nChem., vol. 59, pp. 257-68, 1987.\n[12] M. A. Kabel, M. Maarel, G. Klip, A. Voragen, H. Schols, \"Standard\nassays do not predict the efficiency of commercial cellulase preparations\ntowards plant materials\", Biotechnol. Bioeng., vol. 93(1), pp. 53-63,\n2006."]}
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