Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells
| Autor: | Julian I. Schroeder, Yoshiyuki Murata, Izumi C. Mori, Rayhanur Jannat, Miho Morofuji, Mohammad Muzahidul Islam, C. Robertson McClung, Misugi Uraji, Rachel E. Bloom, Yoshimasa Nakamura |
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| Rok vydání: | 2011 |
| Předmět: |
Physiology
Arabidopsis Gene Expression Plant Science Gene Expression Regulation Enzymologic Article chemistry.chemical_compound Cytosol Gene Expression Regulation Plant Guard cell Enzyme Inhibitors Hydrogen peroxide Abscisic acid Amitrole chemistry.chemical_classification Reactive oxygen species biology Arabidopsis Proteins Protoplasts fungi Wild type food and beverages Hydrogen Peroxide Catalase Cell biology Plant Leaves Mutagenesis Insertional chemistry Biochemistry RNA Plant Plant Stomata Second messenger system biology.protein Reactive Oxygen Species Agronomy and Crop Science Abscisic Acid Signal Transduction |
| Zdroj: | Journal of Plant Physiology. 168:1919-1926 |
| ISSN: | 0176-1617 |
| Popis: | Reactive oxygen species (ROS), including hydrogen peroxide (H(2)O(2)), are among the important second messengers in abscisic acid (ABA) signaling in guard cells. In this study, to investigate specific roles of H(2)O(2) in ABA signaling in guard cells, we examined the effects of mutations in the guard cell-expressed catalase (CAT) genes, CAT1 and CAT3, and of the CAT inhibitor 3-aminotriazole (AT) on stomatal movement. The cat3 and cat1 cat3 mutations significantly reduced CAT activities, leading to higher basal level of H(2)O(2) in guard cells, when assessed by 2',7'-dichlorodihydrofluorescein, whereas they did not affect stomatal aperture size under non-stressed condition. In addition, AT-treatment at concentrations that abolish CAT activities, showed trivial affect on stomatal aperture size, while basal H(2)O(2) level increased extensively. In contrast, cat mutations and AT-treatment potentiated ABA-induced stomatal closure. Inducible ROS production triggered by ABA was observed in these mutants and wild type as well as in AT-treated guard cells. These results suggest that ABA-inducible cytosolic H(2)O(2) elevation functions in ABA-induced stomatal closure, while constitutive increase of H(2)O(2) do not cause stomatal closure. |
| Databáze: | OpenAIRE |
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