Genotyping of Environmental and Clinical Stenotrophomonas maltophilia Isolates and their Pathogenic Potential
Autor: | Thomas Schwartz, Martina Adamek, Josef Winter, Stephan Bathe, Reinhard Fischer, Jörg Overhage |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Heredity
Genotyping Techniques Dictyosteliomycota Stenotrophomonas maltophilia lcsh:Medicine Pathogenesis Polymerase Chain Reaction law.invention law Gram Negative Genome Sequencing Amoeba lcsh:Science Phylogeny Polymerase chain reaction Genetics Multidisciplinary biology Dictyostelium Discoideum Genomics Bacterial Pathogens Phenotype Slime Molds DNA profiling DNA Gyrase Acanthamoeba castellanii Research Article Life sciences Genotypes Virulence Environment Microbiology DNA sequencing Model Organisms ddc:570 Humans Biology Microbial Pathogens Genotyping Protozoan Models Bacterial Taxonomy lcsh:R Computational Biology Genetic Variation Bacteriology Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification DNA Fingerprinting Acanthamoeba bacteria lcsh:Q |
Zdroj: | PLoS ONE, 6 (11), e27615/1-11 PLoS ONE, Vol 6, Iss 11, p e27615 (2011) PLoS ONE |
ISSN: | 1932-6203 |
DOI: | 10.5445/ir/1000027606 |
Popis: | Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequencing distinguished 10 subgroups. For 8 of them, the same strain composition was shown with both typing methods. A subset of 59 isolates representative for the gyrB groups was further investigated with regards to their pathogenic properties in a virulence model using Dictyostelium discoideum and Acanthamoeba castellanii as host organisms. A clear tendency towards accumulation of virulent strains could be observed for one group with A. castellanii and for two groups with D. discoideum. Several virulent strains did not cluster in any of the genetic groups, while other groups displayed no virulence properties at all. The amoeba pathogenicity model proved suitable in showing differences in S. maltophilia virulence. However, the model is still not sufficient to completely elucidate virulence as critical for a human host, since several strains involved in human infections did not show any virulence against amoeba. |
Databáze: | OpenAIRE |
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