Not all tetramer binding CD8+ T cells can produce cytokines and chemokines involved in the effector functions of virus-specific CD8+ T lymphocytes in HIV-1 infected children
| Autor: | Daniel Scott-Algara, Stéphane Blanche, Yves Rivière, Florence Buseyne, Christine Rouzioux, Béatrice Corre, Françoise Porrot, Nassima Bellal |
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| Přispěvatelé: | Biologie des Rétrovirus, Institut Pasteur [Paris], Immunopathologie Virale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Fédération de Pédiatrie, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Infections à Vih, Réservoirs, Pharmacologie des Antirétroviraux et Prévention de la Transmission Mère Enfant, Université Paris Descartes - Paris 5 (UPD5), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ) |
| Rok vydání: | 2004 |
| Předmět: |
MESH : Cytokines
MESH : Gene Products pol MESH : Gene Products gag MESH : HLA-A Antigens HIV Infections MESH : Child Preschool CD8-Positive T-Lymphocytes MESH: HIV-1 Interleukin 21 Epitopes 0302 clinical medicine MESH : Child MESH : Chemokine CCL4 MESH: Child MESH : Chemokine CCL5 Immunology and Allergy Cytotoxic T cell Chemokine CCL4 Child Chemokine CCL5 MESH : Tumor Necrosis Factor-alpha MESH: Cytokines 0303 health sciences ZAP70 MESH : Interferon Type II MESH: HIV Infections MESH : CD8-Positive T-Lymphocytes Macrophage Inflammatory Proteins Natural killer T cell MESH: CD8-Positive T-Lymphocytes 3. Good health MESH: Chemokines CC MESH: Gene Products pol MESH: Gene Products gag Chemokines CC Child Preschool MESH: Oligopeptides Cytokines Oligopeptides MESH : HIV-1 MESH : Oligopeptides MESH: Epitopes Pediatric AIDS Adolescent MESH: Interferon Type II MESH: Macrophage Inflammatory Proteins MESH : Macrophage Inflammatory Proteins Immunology Gene Products gag Gene Products pol MESH : Epitopes Biology CCL5 03 medical and health sciences Interferon-gamma MESH : Adolescent MESH : HIV Infections MESH: Chemokine CCL5 Humans MESH: Chemokine CCL4 Antigen-presenting cell MESH: HLA-A Antigens 030304 developmental biology MESH: Adolescent MESH: Humans HLA-A Antigens Tumor Necrosis Factor-alpha MESH : Humans MESH: Child Preschool Virology MESH: Tumor Necrosis Factor-alpha HIV-1 MESH : Chemokines CC CD8 030215 immunology |
| Zdroj: | Journal of Clinical Immunology Journal of Clinical Immunology, Springer Verlag, 2005, 25 (1), pp.57-67. ⟨10.1007/s10875-005-0358-3⟩ Journal of Clinical Immunology, 2005, 25 (1), pp.57-67. ⟨10.1007/s10875-005-0358-3⟩ Journal of Clinical Immunology, Springer Verlag, 2005, 25 (1), pp.57-67. 〈10.1007/s10875-005-0358-3〉 |
| ISSN: | 0271-9142 1573-2592 |
| Popis: | International audience; In the pediatric human immunodeficiency virus type-1 (HIV-1) infection, the presence of cytotoxic T lymphocytes (CTL) is associated with a slow progression to AIDS. The secretion of cytokines by CTLs may be critical in the control of viral infection. We used the combination of cell surface and intracellular staining to study the functionality of tetramer binding CD8+ T cells recognizing two HIV-1 immunodominant epitopes, in peripheral blood mononuclear cells from HIV-1-infected children. A fraction of tetramer positive CD8+ T cells produce cytokines (IFN-gamma, TNF-alpha) or chemokines (CCL4, CCL5) after ex vivo stimulation with the cognate peptide. There was a negative correlation between the plasma viral load and the percentage of CD8+ Tetramer Gag+ T cells secreting IFN-gamma. This is the first report in the context of pediatric HIV-1 infection showing that only a fraction of HIV-1-specific CD8+ T cells have the capacity to produce cytokines and chemokines implicated in their antiviral functions. |
| Databáze: | OpenAIRE |
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