Laccase and Melanization in Clinically Important Cryptococcus Species Other than Cryptococcus neoformans
| Autor: | Takashi Sugita, Reiko Ikeda, Eric S. Jacobson, Takako Shinoda |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Melanins
Microbiology (medical) Cryptococcus neoformans Laccase Virulence biology Cryptococcus food and beverages Cryptococcosis Mycology Fungi imperfecti biology.organism_classification medicine.disease Microbiology Melanin Biochemistry medicine Humans Oxidoreductases Polyacrylamide gel electrophoresis |
| Zdroj: | Journal of Clinical Microbiology. 40:1214-1218 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.4.1214-1218.2002 |
| Popis: | The laccase enzyme and melanin synthesis have been implicated as contributors to virulence in Cryptococcus neoformans . Since isolations of Cryptococcus species other than C. neoformans from clinical specimens have been increasing, we examined the laccase activities of C. albidus , C. laurentii , C. curvatus , and C. humicola . Incubation of cells with epinephrine produced adrenochrome color in C. albidus , C. laurentii , and C. curvatus but not in C. humicola . Activity was always less than in C. neoformans . Laccase was detected in the soluble fractions of disrupted C. albidus , C. laurentii , and C. curvatus cells. Activity staining of partially purified enzyme after nondenaturing polyacrylamide gel electrophoresis revealed that laccases from C. albidus , C. laurentii , and C. curvatus migrated more slowly than that from C. neoformans . One strain of C. curvatus exhibited two melanin bands. Thus, several clinically emerging Cryptococcus species express laccase and can synthesize melanin. |
| Databáze: | OpenAIRE |
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