Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood
| Autor: | M L Bustos-Bahena, D Correa, Irma Cañedo-Solares, Luz Belinda Ortiz-Alegría, Ricardo Figueroa-Damián, H Luna-Pastén, H González-Henkel, E. Calderon-Segura |
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| Rok vydání: | 2009 |
| Předmět: |
Antibody Affinity
chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Toxoplasmosis Congenital Pregnancy Prenatal Diagnosis Medicine Humans Avidity Serologic Tests Filter paper business.industry Obstetrics and Gynecology medicine.disease Virology Toxoplasmosis Immunoglobulin M Immunoglobulin G Pregnancy Complications Parasitic Pediatrics Perinatology and Child Health Immunology Female business |
| Zdroj: | Journal of perinatology : official journal of the California Perinatal Association. 29(10) |
| ISSN: | 1476-5543 |
| Popis: | To detect immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies and determine immunoglobulin G (IgG) titer and avidity in filter paper-embedded blood (FPEB) samples of pregnant women.A total of 100 FPEB samples of pregnant women (30 positive and 70 negative) were analyzed for anti-T. gondii-specific IgM antibodies. Eleven and nine pairs of serum and FPEB samples were used to standardize IgG titration and avidity, respectively. Then, the correlation of avidity results was determined with 23 serum/FPEB pairs from IgG-positive cases.IgM detection in FPEB was 92% sensitive and 100% specific. The titration of IgG antibodies in FPEB correlated with that of serum (ror=0.9). Significant difference in avidity between the acute and the undetermined/chronic cases was observed in both samples. As expected, no correlation was found between IgM levels and avidity.The FPEB is useful to infer infection phase, and thus to speed clinical decisions in congenital toxoplasmosis management. |
| Databáze: | OpenAIRE |
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