The Effect of TNF-α Blocker HL036337 and Its Best Concentration to Inhibit Dry Eye Inflammation
| Autor: | Wungrak Choi, Hanmil Jang, Yeon Jung Song, Hyemi Noh, Hyea Kyung Ahn, Hyung Keun Lee, Areum Yeo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
genetic structures Microscopy Acoustic Dry eye Inflammation Topical treatment Pharmacology Cornea 03 medical and health sciences Mice 0302 clinical medicine Corneal erosion medicine Effective treatment Animals HL036337 Dose-Response Relationship Drug business.industry Tumor Necrosis Factor-alpha General Medicine eye diseases Mice Inbred C57BL Disease Models Animal 030104 developmental biology Immunology 030221 ophthalmology & optometry Tnf α blockers Tumor necrosis factor alpha Original Article Dry Eye Syndromes Female sense organs medicine.symptom Ophthalmic Solutions business Tumor necrosis factor receptor |
| Zdroj: | Korean Journal of Ophthalmology : KJO |
| ISSN: | 1011-8942 |
| Popis: | Purpose Dry eye syndrome is commonly thought of as an inflammatory disease, and we have previously presented data showing the effectiveness of topical TNF-α blocker agents for the treatment of this condition. The purpose of this study was to investigate the effectiveness of the TNF-α blocking agent HL036337 compared to cyclosporine A for the treatment of dry eye induced inflammation in order to establish whether HL036337 represents a more effective method for suppressing inflammation. The efficacy of HL036337 and cyclosporine A was determined using an experimental murine dry eye model. Methods The TNF-α blocker HL036337 is a modified form of TNF receptor I. Using dry eye induced C57BL/6 mice (n = 45), corneal erosion was measured at day 4 and 7 after topical treatment with cyclosporine A or HL036337. To determine the effective treatment dose, 0.25, 0.5, 1, 2.5, and 5 mg/mL of HL036337 were topically administered twice per day to dry eye induced murine corneas for 1 week. Results The optimal concentration of the TNF-α blocker HL036337 for treatment of dry eye induced corneal erosion was determined to be 1 mg/mL. Dry eye induced corneal erosion was improved after 1 week with topically applied cyclosporine A and HL036337 at 1 mg/mL. Conclusions HL036337 administered topically at 1 mg/mL effectively improved corneal erosion induced by dry eye. This finding may also suggest that inhibition of TNF-α can improve dry eye syndrome. |
| Databáze: | OpenAIRE |
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