The creatine kinase phosphotransfer network: thermodynamic and kinetic considerations, the impact of the mitochondrial outer membrane and modelling approaches
| Autor: | Saks, V., Kaambre, T., Guzun, R., Anmann, T., Sikk, P., Schlattner, U., Wallimann, T., Aliev, M., Marko Vendelin |
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| Přispěvatelé: | Hamant, Sarah, Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics = Keemilise ja bioloogilise füüsika instituut [Estonie] (NICPB | KBFI), Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)-Institute of Cell Biology, Institute of Experimental Cardiology, Cardiology Research Center |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Myocardium
Phosphocreatine MESH: Phosphocreatine Models Biological Mitochondria Heart Adenosine Triphosphate Oxygen Consumption MESH: Adenosine Triphosphate [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Humans MESH: Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Oxygen Consumption Creatine Kinase MESH: Creatine Kinase MESH: Humans MESH: Adenosine Diphosphate MESH: Kinetics Myocardium MESH: Energy Metabolism MESH: Models Biological Adenosine Diphosphate Kinetics Thermodynamics MESH: Mitochondria Heart MESH: Thermodynamics MESH: Mitochondrial ADP ATP Translocases Energy Metabolism Mitochondrial ADP ATP Translocases |
| Zdroj: | Europe PubMed Central Sub-cellular biochemistry Sub-cellular biochemistry, 2007, 46, pp.27-65 Scopus-Elsevier |
| ISSN: | 0306-0225 |
| Popis: | International audience; In this review, we summarize the main structural and functional data on the role of the phosphocreatine (PCr)--creatine kinase (CK) pathway for compartmentalized energy transfer in cardiac cells. Mitochondrial creatine kinase, MtCK, fixed by cardiolipin molecules in the vicinity of the adenine nucleotide translocator, is a key enzyme in this pathway. Direct transfer of ATP and ADP between these proteins has been revealed both in experimental studies on the kinetics of the regulation of mitochondrial respiration and by mathematical modelling as a main mechanism of functional coupling of PCr production to oxidative phosphorylation. In cells in vivo or in permeabilized cells in situ, this coupling is reinforced by limited permeability of the outer membrane of the mitochondria for adenine nucleotides due to the contacts with cytoskeletal proteins. Due to these mechanisms, at least 80% of total energy is exported from mitochondria by PCr molecules. Mathematical modelling of intracellular diffusion and energy transfer shows that the main function of the PCr-CK pathway is to connect different pools (compartments) of ATP and, by this way, to overcome the local restrictions and diffusion limitation of adenine nucleotides due to the high degree of structural organization of cardiac cells. |
| Databáze: | OpenAIRE |
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