Studies on elephant aortic elastic tissue Part I. The histochemistry and fine structure of the fiber
| Autor: | K.G. McCullagh |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
In situ
Pathology medicine.medical_specialty Arteriosclerosis Swine Elephants Clinical Biochemistry Aorta Thoracic Pathology and Forensic Medicine chemistry.chemical_compound medicine Animals Aorta Abdominal Fiber Molecular Biology Orcein Alkaline hydrolysis Aorta Staining and Labeling biology Chemistry Hydrolysis Elastic Tissue Fluorescence Elastin Staining Microscopy Electron Tissue Part biology.protein Biophysics Cattle Spectrophotometry Ultraviolet |
| Zdroj: | Experimental and Molecular Pathology. 18:190-201 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/0014-4800(73)90016-6 |
| Popis: | Elephant arterial elastic laminae were shown to be refractory to staining by orcein or the resorcin dyes, both normally regarded as routine elastic tissue stains. To investigate this more thoroughly, elastin was isolated from arterial tissue by alkaline hydrolysis and studied in vitro . Compared to elastin from other species, elephant elastin was found to resist alkaline hydrolysis to a greater extent, to possess a greater UV absorption at 275 nm, and to show an unusual fluorescence at 415 nm. Electron micrographs of elastic fibres in situ demonstrated the presence of large amounts of a microfibrillar sheath surrounding the amorphous core. These results are interpreted to indicate the presence, in elephant arterial elastic tissue, of unusually large amounts of a nonelastin component which interferes with the normal staining reactions. |
| Databáze: | OpenAIRE |
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