A molecular breadboard: Removal and replacement of subunits in a hepatitis B virus capsid
| Autor: | Elizabeth E. Pierson, Nicholas E. Brunk, Joseph Che Yen Wang, Stephen C. Jacobson, Adam Zlotnick, Panagiotis Kondylis, Martin F. Jarrold, Daniel G. Haywood, David Z. Keifer, Lye Siang Lee |
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| Rok vydání: | 2017 |
| Předmět: |
Boron Compounds
0301 basic medicine Hepatitis B virus Icosahedral symmetry viruses Protein subunit Static Electricity Model system 02 engineering and technology Sodium Chloride medicine.disease_cause Biochemistry Mass Spectrometry Charge detection 03 medical and health sciences Capsid medicine Urea Computer Simulation Molecular Biology Fluorescent Dyes Chemistry Viral Core Proteins Core protein Articles Breadboard 021001 nanoscience & nanotechnology Molecular Weight Protein Subunits Crystallography 030104 developmental biology Ethylmaleimide Biophysics Protein Multimerization 0210 nano-technology Monte Carlo Method |
| Zdroj: | Protein Science. 26:2170-2180 |
| ISSN: | 1469-896X 0961-8368 |
| DOI: | 10.1002/pro.3265 |
| Popis: | Hepatitis B virus (HBV) core protein is a model system for studying assembly and disassembly of icosahedral structures. Controlling disassembly will allow re-engineering the 120 subunit HBV capsid, making it a molecular breadboard. We examined removal of subunits from partially crosslinked capsids to form stable incomplete particles. To characterize incomplete capsids, we used two single molecule techniques, resistive-pulse sensing and charge detection mass spectrometry. We expected to find a binomial distribution of capsid fragments. Instead, we found a preponderance of 3 MDa complexes (90 subunits) and no fragments smaller than 3 MDa. We also found 90-mers in the disassembly of uncrosslinked HBV capsids. 90-mers seem to be a common pause point in disassembly reactions. Partly explaining this result, graph theory simulations have showed a threshold for capsid stability between 80 and 90 subunits. To test a molecular breadboard concept, we showed that missing subunits could be refilled resulting in chimeric, 120 subunit particles. This result may be a means of assembling unique capsids with functional decorations. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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